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To investigate the downstream target gene of YAP1 in enzalutamide-resistant (EnzaR) cells and the effects of extracellular vehicles (EVs) derived from EnzaR cells on enzalutamide-sensitive cells by next-generation sequencing analyses

ABSTRACT: Our study has identified that yes-associated protein 1 (YAP1) is overexpressed in enzalutamide-resistant (EnzaR) cells. Furthermore, enzalutamide-induced YAP1 expression is mediated through the function of chicken ovalbumin upstream promoter transcription factor 2 (COUP-TFII) at the transcriptional and the post-transcriptional levels. Furthermore, both YAP1 and COUP-TFII have proven to be cargoes of EVs by our study. Next, treatment with EnzaR-EVs induces the abilities of enzalutamide resistance in its parental cells while depletion of YAP1 or COUP-TFII in EnzaR-EVs attenuated it. In order to systemically investigate the downstream target gene of YAP1 and the expression profiles changed by EnzaR-EVs from control, YAP1 and COUP-TFII depletions in prostate cancer cells, we have performed RNA-seq analyses to pursuit the research directions of those questions.

ORGANISM(S): Homo sapiens

PROVIDER: GSE164880 | GEO | 2024/01/13

REPOSITORIES: GEO

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Expression data from control and COUP-TFII siRNA treated PC3 cells

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Project description:The orphan nuclear receptor COUP-TFII is expressed at a low level in adult tissues, but its expression is increased and shown to promote progression of multiple diseases including prostate cancer, heart failure and muscular dystrophy. Suppression of COUP-TFII slows disease progression, making it an intriguing therapeutic target. Here, we identified a potent and specific COUP-TFII inhibitor through high-throughput screening. The inhibitor specifically suppressed COUP-TFII activity to regulate its target genes.

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