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Gene expression microarray analysis of whole mouse hamstring muscles to identify genes promoting the devlopment of neurogenic heterotopic ossifications following spinal cord injury

ABSTRACT: To better understand which genes/pathways are activated in injured muscles following a spinal cord injury (SCI) and how these promote the development of neurogenic heterotopic ossification (NHO), we performed gene expression profiling by microarray of muscles in the early phase following muscle injury.

ORGANISM(S): Mus musculus

PROVIDER: GSE165062 | GEO | 2021/12/01

REPOSITORIES: GEO

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Real-time quantitative PCR analysis of microRNAs in a mice model of Neurogenic Heterotopic Ossifications (NHOs)

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Interorgan communication in the neurogenic heterotopic ossification: focusing on the role of brain-derived extracellular vesicles

Project description:Brain-derived extracellular vesicles participate in inter-organ communication after traumatic brain injury by transporting pathogens to initiate secondary injury. Inflammasome related proteins encapsulated in brain-derived extracellular vesicles are capable of crossing the blood-brain barrier to reach distal tissues. These proteins initiate inflammatory dysfunction such as neurogenic heterotopic ossification. This recurrent condition is highly debilitating to patients because of its relatively unknown pathogenesis and the lack of effective prophylactic intervention strategies. Accordingly, a rat model of neurogenic heterotopic ossification that combined traumatic brain injury and achillotenotomy was developed to address these two issues. Histological examination of the injured tendon identified the coexistence of ectopic calcification and fibroblast pyroptosis. The relationships among brain-derived extracellular vesicles, fibroblast pyroptosis and ectopic calcification were further investigated in vitro and in vivo. Intravenous injection of the pyroptosis inhibitor Ac-YVAD-cmk reversed the development of neurogenic heterotopic ossification in vivo. The present work highlighted the role of brain-derived extracellular vesicles in the pathogenesis of neurogenic heterotopic ossification and offered a potential strategy for the prevention of neurogenic heterotopic ossification after traumatic brain injury.

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Gene expression microarray analysis of whole mouse hamstring muscles to identify genes promoting the devlopment of neurogenic heterotopic ossifications following spinal cord injury

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Project description:Heterotopic ossification (HO), the pathologic growth of extra-skeletal bone, occurs as a common complication of trauma or in genetic disorders and can be disabling and lethal.We report that mice harboring injury-induced HO exhibit bone loss similar to the presentation of HO patients. Esepecially, Fetuin A dysregulation promotes immunosupression in HO lesion to link HO and bone loss.

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