Dataset Information

Transcriptome sequencing revealed a set of differentially expressed related genes between systemic lupus erythematosus (SLE) patients with and without pulmonary fibrosis (PF)

ABSTRACT: Systemic lupus erythematosus (SLE) is an autoimmune disease characterized by abnormal immune responses that include aberrant activation of autoreactive T and B lymphocytes, with subsequent production of pathogenic autoantibodies and formation of immune complexes leading to organ injury. Pulmonary involvement in SLE is common and protean with different clinical manifestations ranging from acute pneumonitis, pulmonary hemorrhage to pulmonary fibrosis (PF). The health status of SLE patients with pulmonary disease is relatively poor, and the mortality rate is high, but research on SLE pulmonary domain is still lacking. In order to further explore the difference between SLE with PF and SLE without PF, we perform transcriptome sequencing analysis. The PBMCs from 3 SLE patients with PF and 3 SLE without PF were drawn, and RNA-seq was performed with subsequent bioinformatics analysis. Compared with the SLE patients without PF, the genes that changed in all three SLE patients with PF included 647 significantly up-regulated and 1364 significantly down-regulated differentially expressed genes.

ORGANISM(S): Homo sapiens

PROVIDER: GSE165159 | GEO | 2024/01/20

REPOSITORIES: GEO

ACCESS DATA

Similar Datasets

Project description:Objective: Pulmonary complications in systemic sclerosis (SSc), including pulmonary fibrosis (PF) and pulmonary arterial hypertension (PAH), are the leading cause of mortality. We compared the molecular fingerprint of SSc lung tissues and matching primary lung fibroblasts to those of normal donors, and patients with idiopathic pulmonary fibrosis (IPF) and idiopathic pulmonary arterial hypertension (IPAH). Methods: Lung tissues were obtained from 33 patients with SSc who underwent lung transplantation. Tissues and cells from a subgroup of SSc patients with predominantly PF or PAH were compared to those from normal donors, patients with IPF, or IPAH. Microarray data was analyzed using Efficiency Analysis for determination of optimal data processing methods. Real time PCR and immunohistochemistry were used to confirm differential levels of mRNA and protein, respectively. Results: We identified a consensus of 242 and 335 genes that were differentially expressed in lungs and primary fibroblasts, respectively. Enriched function groups in SSc-PF and IPF lungs included fibrosis, insulin-like growth factor signaling and caveolin-mediated endocytosis. Functional groups shared by SSc-PAH and IPAH lungs included antigen presentation, chemokine activity, and IL-17 signaling. Conclusion: Using microarray analysis on carefully phenotyped SSc and comparator lung tissues, we demonstrated distinct molecular profiles in tissues and fibroblasts of patients with SSc-associated lung disease compared to idiopathic forms of lung disease. Unique molecular signatures were generated that are disease- (SSc) and phenotype- (PF vs PAH) specific. These signatures provide new insights into pathogenesis and potential therapeutic targets for SSc lung disease. Lung tissues were obtained from 33 patients with SSc who underwent lung transplantation. Tissues and cells from a subgroup of SSc patients with predominantly PF or PAH were compared to those from normal donors, patients with IPF, or IPAH. Microarray data was analyzed using Efficiency Analysis for determination of optimal data processing methods. Real time PCR and immunohistochemistry were used to confirm differential levels of mRNA and protein, respectively.

Dataset Information

Transcriptome sequencing revealed a set of differentially expressed related genes between systemic lupus erythematosus (SLE) patients with and without pulmonary fibrosis (PF)

Similar Datasets

OmicsDI is part of the ELIXIR infrastructure