Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Characterization of the adaptive transcriptional response triggered by the cell wall interfering compound Poacic acid in yeast and its effect on crosslinking enzymes.

ABSTRACT: We analyzed the genome-wide expression profile of the wild type strain in response to poacid acid (1 hour of treatment). In addition, we did transcription profiling on the effect of slt2 (MAPK of Cell wall intregity pathway) deletionin genes involved in poacid acid response

ORGANISM(S): Saccharomyces cerevisiae

PROVIDER: GSE165530 | GEO | 2021/04/25

REPOSITORIES: GEO

ACCESS DATA

Json Xml

Similar Datasets

Characterization of the adaptive transcriptional response triggered by the cell wall interfering compound Poacic acid in yeast and its effect on crosslinking enzymes.

Project description:Characterization of the adaptive transcriptional response triggered by the cell wall interfering compound Poacic acid in yeast and its effect on crosslinking enzymes.

| PRJNA694910 | ENA

Saccharomyces cerevisiae

Project description:slt2 mutant 3 hour exposure to zymolyase

| PRJNA114687 | ENA

Chemically triggered crosslinking with bioorthogonal cyclopropenones.

Project description: Not available

| S-EPMC7501174 | biostudies-literature

The role of the Hog1 and Slt2 MAP Kinases in the regulation of Saccharomyces cerevisiae gene expression upon stress by sulfuric acid

Project description:In our previous work, we had found that Saccharomyces cerevisiae needs of the Hog1 and Slt2 proteins to growth in a low pH environment caused by sulfuric acid, one of the stress factors during the process of ethanol production. Then was performed the gene-wide expression analysis in the hog1∆ and slt2∆ mutants in order to reveal the function of the Hog1p and Slt2p MAP Kinases in the regulation of S. cerevisiae global gene expression upon stress by sulfuric acid.

2013-10-18 | GSE51353 | GEO

Leukotriene D4-triggered Transcriptome

Project description:Human umbilical vein endothelial cells (HUVEC) or the human macrophage cell line, Mono-Mac-6, treated with Leukotriene D4 for 1 hour Keywords = Leukotriene-Transcriptome Keywords = Mono-Mac-6 Keywords = HUVEC Keywords: other

2004-08-20 | GSE1644 | GEO

Engineering acyl-homoserine lactone-interfering enzymes toward bacterial control.

Project description: Not available

| S-EPMC7489903 | biostudies-literature

Super-Resolution Microscopy and Single-Molecule Tracking Reveal Distinct Adaptive Dynamics of MreB and of Cell Wall-Synthesis Enzymes.

Project description: Not available

| S-EPMC7468405 | biostudies-literature

Sodium arsenate response of wild type and slt2 deficient cells

Project description:Wild type (BY4741) Saccharomyces cerevisiae strains and their isogenic slt2 deficient counterparts, were treated for 2 hours with sodium arsenate 100 micromolar. Control (untreated) cells were also collected. Total RNA was extracted and analyzed by microarray hybridization. The data obtained from these experiments allows to determine those genes that are regulated by Slt2 activity after arsenate treatment. The experiments was designed as follows: Four types of samples were used: untreated wild type cells, treated wild type cells, untreated slt2- cells and treated slt2- cells. 3 independent replicates were performed for each experiment.

2010-07-25 | E-GEOD-17364 | biostudies-arrayexpress

Investigating the mechanism by which the natural product compound puupehenone potentiates the antifungal drug caspofungin

Project description:The cell wall-targeting echinocandin antifungals, although potent and well-tolerated, are inadequate in treating fungal infections due to their narrow spectrum of activity and their propensity to induce pathogen resistance. A promising strategy to overcome these drawbacks is to combine echinocandins with a molecule that improves their activity and also disrupts drug adaptation pathways. In this study, we show that puupehenone (PUUP), a marine sponge-derived sesquiterpene quinone potentiates the echinocandin drug, caspofungin (CAS) in CAS-resistant fungal pathogens. We have conducted RNA-Seq analysis, followed by genetic and molecular studies, to elucidate PUUP’s CAS-potentiating mechanism. We found that the combination of CAS and PUUP blocked the induction of CAS-responding genes required for the adaptation to cell wall stress through the cell wall integrity (CWI) pathway. Further analysis showed that PUUP inhibited the activation of Slt2 (Mpk1), the terminal MAP kinase in this pathway. We also found that PUUP induced heat shock response genes and inhibited the activity of heat shock protein 90 (Hsp90). Molecular docking studies predicted that PUUP occupies a binding site on Hsp90 required for the interaction between Hsp90 and its co-chaperone Cdc37. Thus, we show that PUUP potentiates CAS activity by a previously undescribed mechanism which involves disruption of Hsp90 activity and the CWI pathway. Given the requirement of the Hsp90-Cdc37 complex in Slt2 activation, we suggest that inhibitors of this complex would disrupt the CWI pathway and synergize with echinocandins. Therefore, the identification of PUUP’s CAS-potentiating mechanism has important implications in the development of new antifungal combination therapies.

2019-12-04 | GSE140563 | GEO

Enzyme-triggered compound release using functionalized antimicrobial peptide derivatives.

Project description: Not available

| S-EPMC5380883 | biostudies-literature