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Qualitative changes in activated T cells induced by checkpoint blockade in vivo

ABSTRACT: Here we perform QuantSeq 3' mRNA sequencing of RNA extracted from flow sorted splenic T cell from Tg4 Nr4a3-Tocky Tiger mice that had been immunised with 80ug of 4Y MBP peptide for 24 hours. After 24 hours mice were re-stimulated with 8ug 4Y MBP in the presence of isotype, anti-PD1 or anti-Lag3 antibodies for 4 hours. The results show that anti-PD1 but not anti-Lag3 imparts a unique transcriptional signature on responding T cells, indicative of stronger TCR signalling.

ORGANISM(S): Mus musculus

PROVIDER: GSE165818 | GEO | 2021/07/30

REPOSITORIES: GEO

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