Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Epigenetic regulation of gene expression in mouse skin after ionizing radiation damage

ABSTRACT: Back skin from 8-10 weeks male mice was plucked to induce actively growing hair follicles. After 9 days, the back skin was irradiated with 5Gy ionizing radiation. Skin samples were collected for CHIP-seq analysis using a p53 antibody and H3K4me3 antibody. We compared wild type and Krt17 knock out mice for their epigenetic regulation of gene expression change in response to ionizing radiation

ORGANISM(S): Mus musculus

PROVIDER: GSE165840 | GEO | 2021/01/31

REPOSITORIES: GEO

ACCESS DATA

Json Xml

Similar Datasets

Ionizing radiation induced hair and skin damage

Project description:Skin samples from back region and footpads were analyzed in 8-10 week old mice. For back skin, the hair was plucked in order to induce active growing phase(anagen). After 9 days, the mice were irradiated for 5Gy IR. Krt17 knock out, p53 knock out, and wild type mice were compared. Samples were taken at time 0 (control), 6h, and 24h post-irradiation. For footpad skin, the mice were irradiated for 40 Gy IR. Krt17 knock out and wild type mice were compared. Samples were taken at time 0 (control), 3 d, 6 d, 9 d post-irradiation.

2020-12-22 | GSE163653 | GEO

IMR90 DDR

Project description:Transcription profiling of IMR90 fibroblasts after ionizing radiation. Cells were harvested 30 minutes (acute response) or 5 days (cell cycle arrested) after being irradiated with 5Gy of ionizing radiation.

2014-06-17 | E-MTAB-2088 | biostudies-arrayexpress

Ionizing radiation induced hair and skin damage

Project description:Ionizing radiation induced hair and skin damage

| PRJNA687009 | ENA

Plucked scalp hair follicle samples are useful RNA sources for mRNA analysis of genodermatosis-associated genes

Project description:Using RNA samples extracted from plucked hair follicles and skin samples, we conducted comprehensive RNA sequencing and evaluated expression levels of 347 genes causative of genodermatoses.

2023-06-19 | GSE224010 | GEO

CNA analysis of ionizing radiation related basal cell carcinoma

Project description:gDNA from patient samples with multiple basal cell carcinomas and possible exposure to ionizing radiation was hybridized Vs. GM12878 gDNA to assess CNAs. We aimed to find a possible common aberration pattern related to ionizing radiation or a rare metastasis.

2017-10-02 | GSE97334 | GEO

Dose- and time- dependent ionizing ratidation effect on mice peripheral blood

Project description:Gene expression profiles of peripheral blood samples from C57BL/6 mice exposed with ionizing radiation. We used mice as model animal to study biologial recovery response after radiation damage. Therefore, we obtained gene expression profiles from C57BL/6 mice exposed with various levels of ionizing radiation, including low and high doses and control groups. In order to measure recovery rate, we collected peripheral blood samples at different time durations after the exposure. In order to obtain robust signatures specific to radiation response, we are interested in knowing if the radiation signarures will be present in the presence of confounders. Therefore, mice were given intraperitoneal injections of lipopolysaccharide endotoxin (LPS), or treated with granulocyte colony-stimulating factor (GCSF), otherwise no treatment after ionizing radiation exposure. The underlying mechanism of confounder treatment is that LPS induces strong immune response resembling the effect of infection, and GCSF stimulates mobilization of HSCs. Exploratory analysis shows that the confounding effects did affect the radiation signature to some extent. This study provides insights into the molecular basis of time- and dose- dependent response to ionizing radiation in mice hematopoietic system.

2013-11-15 | GSE52403 | GEO

Dose- and time- dependent ionizing ratidation effect on mice peripheral blood

2013-11-15 | E-GEOD-52403 | biostudies-arrayexpress

Ionizing Radiation-induced expression response in Drosophila Larvae

Project description:Genome-wide expression analysis comparison with and without ionizing radiation in p53 mutant and wild type Drosophila larvae

2012-04-18 | GSE37404 | GEO

Densely Ionizing Radiation Effects on the Microenvironment Promote Aggressive Trp53 Null Mammary Carcinomas

Project description:Densely ionizing radiation is a major component of the space radiation environment and has potentially greater carcinogenic effect compared to sparsely ionizing radiation that is prevalent in the terrestrial environment. It is unknown to what extent the irradiated microenvironment contributes to the differential carcinogenic potential of densely ionizing radiation. To address this gap, 10-week old BALB/c mice were irradiated with 100 cGy sparsely ionizing g-radiation or 10, 30, or 80 cGy of densely ionizing, 350 MeV/amu Si particles and transplanted 3 days later with syngeneic Trp53 null mammary fragments. Tumor appearance was monitored for 600 days. Tumors arising in Si-particle irradiated mice had a shorter median time to appearance, grew faster and were more likely to metastasize. Most tumors arising in sham-irradiated mice were ER-positive, pseudo-glandular and contained both basal keratin 14 and luminal keratin 8/18 cells (designated K14/18), while most tumors arising in irradiated hosts were K8/18 positive (designated K18) and ER negative. Comparison of K18 vs K14/18 tumor expression profiles showed that genes increased in K18 tumors were associated with ERBB2 and KRAS while decreased genes overlapped with those down regulated in metastasis and by loss of E-cadherin. Consistent with this, K18 tumors grew faster than K14/18 tumors and more mice with K18 tumors developed lung metastases compared to mice with K14/18 tumors. However, K18 tumors arising in Si-particle irradiated mice grew even faster and were more metastatic compared to control mice. A K18 Si-irradiated host profile was enriched in genes involved in mammary stem cells, stroma, and Notch signaling. Thus systemic responses to densely ionizing radiation enriches for a ER-negative, K18-positive tumor, whose biology is more aggressive compared to similar tumors arising in non-irradiated hosts. Key Words: ionizing radiation; breast cancer; heavy ion radiation;initiation; promotion

2014-10-10 | GSE56704 | GEO

Possible DNA damage after paternal exposure to ionizing radiation in radar technicians

Project description:Effects of accidental and prolonged radiation exposure on the human germline remain a topic of medical interest. Transgenerational signatures of ionizing radiation exposure are primarily implicated to be clustered de-novo mutations (cDNMs, multiple de-novo mutations within 20bp). We sequenced the whole genome of 110 children from 70 families (Radar cohort). The father in this cohort were exposed to 0-353mSv of ionizing radiation and serve as exposed samples.

| EGAS00001007321 | EGA

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data