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Kruppel-like factor 2 is important for endoderm-related gene regulation with Ring1b-mediated mechanism in mESCs

ABSTRACT: We report the RNA-seq data of Klf2 KO and Ring1b KD from mouse embryonic stem cells. Wild type and Klf2 knockout cells were knockdowned with Ring1b shRNA. Samples were lysed with total RNA prep kit.

ORGANISM(S): Mus musculus

PROVIDER: GSE165873 | GEO | 2021/02/01

REPOSITORIES: GEO

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Discovery of Klf2 Interactors in Mouse Embryonic Stem Cells by Immunoprecipitation-Mass Spectrometry Utilizing Exogenously Expressed Bait

Project description:We report the RNA-seq data of Klf2 knockdowned cells and Snd1 knockdowned cells from mouse embryonic stem cells. Samples were lysed with total RNA prep kit.

2021-03-24 | GSE169406 | GEO

Kruppel-like factor 2 is important for endoderm-related gene regulation with Ring1b-mediated mechanism in mESCs

Project description:Kruppel-like factor 2 is important for endoderm-related gene regulation with Ring1b-mediated mechanism in mESCs

| PRJNA698396 | ENA

Catalytically inactive Ring1B maintains near wildtype levels of gene expression in mESCs

Project description:This experiment was designed to determine the extent of gene misregulation in mESCs containing catalytically dead Ring1B in comparison to mESCs lacking Ring1B.

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Catalytically inactive Ring1B maintains near wildtype levels of gene expression in mESCs

Project description:This experiment was designed to determine the extent of gene misregulation in mESCs containing catalytically dead Ring1B in comparison to mESCs lacking Ring1B. Polyadenylated mRNA was prepared from wildtype mESCs (WT), mESCs deficient for Ring1B (KO) and mESCs cells containing catalytically dead Ring1B (I53A). Each sample is represented by 3 biological replicate hybridisations.

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Mouse ES cells expressing catalytically inactive Ring1B display impaired Ring1B and H3K27me3 deposition.

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Project description:Polycomb repressive complex PRC1 is essential for gene regulation in numerous cell fate decisions. We show that RING1B (RING2, RNF2) and canonical PRC1 (cPRC1) genes are amplified and overexpressed in breast cancer (BC). Moreover, cPRC1 complexes functionally associate with genes regulated by cell type specific key transcription factors such as estrogen receptor (ER) in ER+ tumor cells and BRD4 in triple negative BC cells. cPRC1 is recruited to active enhancers in a manner independent of PRC2 and RING1B enzymatic activity. RING1B regulates enhancer activity and gene transcription not only by promoting the expression of BC oncogenes but also by regulating chromatin accessibility for oncogenic transcription factors. RING1B recruitment, and thus PRC1 association, to active enhancers occurs in multiple cancers.

2018-10-19 | PXD009570 | Pride

Mouse ES cells expressing catalytically inactive Ring1B display impaired Ring1B and H3K27me3 deposition.

Project description:ChIP-seq for H3K27me3 and Ring1B was performed in WT mESCs and mESCs containing catalytically inactive Ring1B (I53A mutant). Cells expressing catalytically inactive Ring1B maintain the spatial distribution of Ring1B and H3K27me3 but at reduced levels. These findings support the notion that PRC2 recruitment is, in part, dependent on H2A ubiquitination (H2AK119ub). Two biological replicates were performed for Ring1B and H3K27me3 ChIPs in WT and Ring1B I53A/I53A mouse ESCs. Input chromatin was sequenced for each replicate as a control for ChIP enrichment.

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RING1B binding in the 11 dpc embryonic tissues

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Kruppel-like factor 2+ CD4 T cells avert microbiota induced intestinal inflammation [RNA-seq]

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RING1B calibrated ChIP-seq (cChIPseq) datasets for control mESCs and an Scc1-AID auxin-inducible degron.

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