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Transcriptomic analysis of an E. coli deletion mutant of the two C-terminal OB fold domains of r-protein S1

ABSTRACT: Escherichia coli ribosomal protein S1 is essential for cellular viability through translation initiation of mRNAs. Here, we have addressed the roles of the two distal OB fold domains located in the C-terminus, which are dispensable for growth. Their deletion causes a cold-shock phenotype and a loss of motility accompanied by deregulation of the expression of several small regulatory RNAs (sRNAs) and many mRNAs involved in stress responses. Surprisingly, the expression of the sRNA RyhB and one of its repressed target mRNA, sodB, were found enhanced in the mutant strain. We used a combination of in vivo and in vitro approaches to address the roles of S1 in RyhB-dependent regulation. We found that the RyhB-dependent repression of sodB translation was not altered but instead rapid RNase E-mediated depletion of the repressed sodB mRNA was strongly impaired in the mutant strain. This unexpected effect was also generalized to other RyhB-dependent targets. In addition, deletion of the last two OB domains of S1 causes a defect of the final step of the RNase E-dependent maturation of the 16S rRNA. This work unveils an unexpected function of S1 in enhancing the kinetics of ribosome biogenesis and RyhB-dependent mRNA decay mediated by the RNA degradosome. Through its RNA chaperone activity, S1 facilitates the existing coupling between ribosome biogenesis, translation, and RNA decay.

ORGANISM(S): Escherichia coli str. K-12 substr. MG1655

PROVIDER: GSE166046 | GEO | 2022/07/31

REPOSITORIES: GEO

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