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Single Cell Transcriptomic Analysis of hiPSC-lt-NES cells

ABSTRACT: We performed single-cell RNA-seq of human iPSC-derived long-term self-renewing neural epithelial stem cells (hiPSC-lt-NES cells) using Quartz-seq methods to characterize cellular heterogeneity .

ORGANISM(S): Homo sapiens

PROVIDER: GSE166056 | GEO | 2023/06/04

REPOSITORIES: GEO

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Transcriptome analysis of single cell-derived hiPSC-lt-NES cell clones

Project description:To identify cell-populations within human iPSC-derived long-term self-renewing neural epithelial stem cells (hiPSC-lt-NES cells) which retain capacities to generate undesired grafts, we established single cell-derived hiPSC-lt-NES cell clones and performed gene expression microarray analysis.

2023-06-04 | GSE166051 | GEO

Transcriptome analysis of hiPSC derived lt-NES cells

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Project description:CHARGE syndrome is a congenital disorder caused by mutations in Chromodomain Helicase DNA-binding domain 7 (CHD7) gene. We performed single cell RNA-seq analysis in CTRL and CHD7-knockdown lt-NES cells.

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Analysis of gene expression profile in the control and CHD7-knockdown hiPSC-derived lt-NES cells (scRNA-Seq)

Project description:Analysis of gene expression profile in the control and CHD7-knockdown hiPSC-derived lt-NES cells (scRNA-Seq)

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Robust production of human neural cells by establishing neuroepithelial-like stem cells from peripheral blood mononuclear cell-derived feeder-free iPSCs under xeno-free conditions

Project description:We performed a microarray experiment to analyze the transcriptional profile of human fetal tissue derived neural stem/progenitor cells, human iPSCs, or human iPSC-derived neural stem/progenitor cells generated using xenogenic or xeno-free reagents. Gene expression patterns were compared among human fetal tissue derived neural stem/progenitor cells, human iPSCs, or human iPSC-derived neural stem/progenitor cells generated using xenogenic or xeno-free reagents. Samples with a title including "Xf" (201B7-lt-NES (Xf)-1, 201B7-lt-NES (Xf)-2, 1210B2-lt-NES (Xf)-1,1210B2-lt-NES (Xf)-2, 1231A3-lt-NES (Xf)-1, 1231A3-lt-NES (Xf)-2) correspond to human iPSC-derived neural stem/progenitor cells generated using xeno-free reagents, whereas samples with a title including "Con" (201B7-lt-NES (Con)-1, 201B7-lt-NES (Con)-2) correspond to those generated using xenogenic reagents.

2016-08-22 | E-GEOD-76900 | biostudies-arrayexpress

Analysis of single cell gene expression profile in human iPSC-derived lt-NES cells

Project description:Analysis of single cell gene expression profile in human iPSC-derived lt-NES cells

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Single cell RNA-seq of the human long-term self-renewing neuroepithelial-like stem cell line SAI2

Project description:The transcriptome of single long-term self-renewing neuroepithelial-like stem cells (LT-NES) was studied in order to establish a baseline for investigations into modules of synergistically active transcription factors that determine developmental cell subpopulations

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Analysis of single cell gene expression profile in human iPSC-derived lt-NES cells

Project description:We performed single-cell total RNA-seq of iPSC-derived neuroepitherial-like stem cells using RamDA-seq methods. We observed hetelogeneity and classified it based on differentiation potential.

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Impact of Zika Virus Infection on Human Neural Stem Cell MicroRNA Signatures [array]

Project description:We used whole genome expression profiling to analyze the effects of Zika virus (ZIKV) strains Uganda and French Polynesia on the mRNA transcriptome of induced pluripotent stem cell-derived human neural stem cells (long-term self-renewing neuroepithelial-like stem (lt-NES®) cells) - particularly to identify differentially expressed genes which are associated with microcephaly phenotype.

2020-10-31 | GSE157530 | GEO

Notch engagement by Jag1 clusters on the nanoscale suggests a force-independent mode of activation

Project description:The Notch signaling pathway is a cell-cell communication system with fundamental roles in embryonic development and the nervous system, including neural stem cell proliferation and differentiation. To investigate the multivalency effect of ligands on the activation of the Notch receptor, we treated iPSc-derived neuroepithelial stem-like (lt-NES) cells with different Jag1 nanopatterns on DNA origami nanostructures.

2023-11-23 | E-MTAB-12439 | biostudies-arrayexpress

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