Differential gene expression analysis on cells expressing Wild type GFP-MAB21L1 and its mutant form GFP-Arg51Leu and GFP-Arg51Gln

ABSTRACT: RNA Sequencing analysis was performed on RNA isolated from cells tagged with GFP-MAB21L1 and its mutant form GFP-Arg51Leu and GFP-Arg51Gln with and without treatment of tetracycline for 12 hrs. Transcript-level quantitation was performed using Salmon (v0.8.2) against the GRCh38 Ensembl reference transcriptome (release-89).Transcript-level counts were summarized to gene level using the bioconductor package tximport (v1.4.0). Differential expression analysis was performed with the bioconductor package DESeq2 (v1.30.0) using the Wald significance tests.

ORGANISM(S): Homo sapiens

PROVIDER: GSE166078 | GEO | 2021/11/27

REPOSITORIES: GEO