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Single cell sequencing on pediatric sialoblastoma

ABSTRACT: As a rare embryonal malignant tumor, sialoblastoma recapitulates primitive salivary gland anlage, usually locating in parotid or submandibular gland and diagnosed at birth or during infancy. The transcriptome profiles as well as intertumoral heterogeneity of sialoblastoma have not yet been investigated. Here, through single-cell RNA sequencing of tumor samples from the same patient before and after chemotherapy, we constructed the first cell transcriptomic atlas of sialoblastoma. By calculating differentially expressed genes and performing gene ontology analysis, we found downregulation of cell proliferation while upregulation of epidermic differentiation pathways after chemotherapy. In addition, we demonstrated distinct transcriptional programs embedded in tumor, including cycling cells, basaloid cells, ductular cells, myoepithelial-like cells and transitional states. The proportion of cycling cells and transitional state cells decreased after chemotherapy whereas the proportion of basaloid cells and myoepithelial cells increased. Taken together, we offered a valuable resource for deciphering cellular heterogeneity as well as adaptive change of tumor cells after chemotherapy, providing insights into clinical management of sialoblastoma.

ORGANISM(S): Homo sapiens

PROVIDER: GSE166345 | GEO | 2022/07/04

REPOSITORIES: GEO

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