Genome wide mapping of DNA lesions by Repair Assisted Damage Detection sequencing – RADD-Seq

ABSTRACT: This method, termed Repair Assisted Damage Detection sequencing (RADD-seq), uses repair enzymes to excise the DNA lesions leaving a single-strand gap. The damage sites are then repaired in-vitro with biotinylated nucleotides, followed by DNA fragmentation, immunoprecipitation and sequencing. Reads are mapped back to the reference genome, indicating the locations of damage sites.

ORGANISM(S): Homo sapiens

PROVIDER: GSE166843 | GEO | 2021/02/17

REPOSITORIES: GEO