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ACME dissociation: a versatile cell fixation-dissociation method for single-cell transcriptomics

ABSTRACT: We performed single-cell transcriptome analysis (using 10x genomics 3' scRNA-seq v3.1 chemistry) in the sea anemone Nematostella vectensis (Cnidaria).

ORGANISM(S): Nematostella vectensis

PROVIDER: GSE166977 | GEO | 2021/04/01

REPOSITORIES: GEO

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Post transcriptional modifications of miRNAs in the cnidarian Nematostella vectensis

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Decoupling behavioral and transcriptional responses to color in an eyeless cnidarian

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Project description:NvNcol3::mOrange2 is a stable transgenic line that labels cnidocytes(stinging cells) of the sea anemone Nematostella vectensis (Nakanishi et al., Development 2012). Two week old primary polyps were dissociated and the NvNcol3::mOrange2 positive and negative cells were enriched by FACS.

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The chromatin-regulating CoREST complex is animal specific and essential for development in the cnidarian Nematostella vectensis

Project description:Using the sea anemone Nematostella vectensis as a model for early branching metazoans, we show that CoREST is an animal-specific protein that assembles a conserved histone-modifying complex including Lsd1 and HDAC1/2. We further show that the Nematostella complex is similar in composition to vertebrates.

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MAPK signaling promotes embryonic neural induction and expression of embryonic patterning genes in Nematostella vectensis

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