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ChIP-seq of Streptococcus pneumoniae D39 metR over-expression strain with 3×flag tag in CDM without methionine

ABSTRACT: In our study, we found that MetR as a transcriptional regulator may regulate the expression of genes not involved in methionine synthesis. To verify our hypothesis, we firstly construct a strain with metR over-expression in D39 and with 3×flag tag and culture this strain in chemically defined medium (CDM) without methionine. Then collect bacteria for ChIP-seq to identify the genes that can be directly transcriptionally regulated by MetR.

ORGANISM(S): Streptococcus pneumoniae D39

PROVIDER: GSE167148 | GEO | 2021/05/18

REPOSITORIES: GEO

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