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Comparison of gene expression in the salmon parr precocious and non-precocious testis

ABSTRACT: The objective was to compare gene expression in salmon precocious and non-precocious parr testis tissue.

ORGANISM(S): Salmo salar Oncorhynchus mykiss

PROVIDER: GSE16721 | GEO | 2009/12/31

SECONDARY ACCESSION(S): PRJNA122737

REPOSITORIES: GEO

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Comparison of gene expression in the salmon parr precocious and non-precocious testis

Project description:The objective was to compare gene expression in salmon precocious and non-precocious parr testis tissue. Nine microarray hybridisations were carried out in total as part of this loop design. Total RNA from 3 pools(PG1-PG3) each of 4 precocious fish testes were compared with 3 pools (NP1-NP3) each of 10 non-precocious fish testes. There were no direct dye swaps but there was overall dye balance in the loop

2010-05-16 | E-GEOD-16721 | biostudies-arrayexpress

Comparison of precocious and non-precocious salmon brain

Project description:The objective was to compare gene expression in salmon precocious and non-precocious parr brain tissue.

2009-12-31 | GSE16720 | GEO

Comparison of precocious and non-precocious salmon

Project description:This SuperSeries is composed of the following subset Series: GSE16720: Comparison of precocious and non-precocious salmon brain GSE16721: Comparison of gene expression in the salmon parr precocious and non-precocious testis Refer to individual Series

2010-05-16 | E-GEOD-16860 | biostudies-arrayexpress

Comparison of precocious and non-precocious salmon brain

Project description:The objective was to compare gene expression in salmon precocious and non-precocious parr brain tissue. Eight microarray hybridisations were carried out in total as part of this design. To compare brain samples total RNA from 10 individual brains (dissected of hypothalamus and pituitary) of precocious fish and 10 brains of non-precocious fish were pooled to give two pools each of five precocious (PA and PB) and non-precocious (NPA and NPB) brains. Each pool of precocious brains was compared in a dye swap experiment with each pool of non-precocious brains.

2010-05-16 | E-GEOD-16720 | biostudies-arrayexpress

Gene expression in liver, gill, hypothalamus, pituitary, and olfactory rosettes from Atlantic salmon parr and smolt

2012-08-20 | GSE33711 | GEO

Gene expression in liver, gill, hypothalamus, pituitary, and olfactory rosettes from Atlantic salmon parr and smolt

Project description:To identify genes involved in the developmental process of Atlantic salmon smoltification, gene expression was compared between smolt and parr in tissues involved in osmoregulation (gill), metabolism (liver), imprinting (olfactory rosettes) and neuroendocrine control (hypothalamus and pituitary). Tissue samples were harvested from laboratory-reared parr and smolts on the same date. Smolts were distinguished from parr by size and appearance; developmental status was confirmed by physiological assays. Eight biological replicates (16 fish) balanced for sex and for dye were used in the liver, gill, olfactory rosette, and hypothalamus comparisons. Four male parr were compared to four male smolts and four female parr were compared to four female smolts; smolts were labeled with Alexa Fluor 555 on four arrays and with Alexa Fluor 647 on four arrays. Six biological replicates (12 fish) were used for the pituitary comparison (two female and four male).

2012-08-20 | E-GEOD-33711 | biostudies-arrayexpress

Salmo salar

Project description:Comparison of gene expression in the salmon parr precocious and non-precocious testis

| PRJNA122737 | ENA

Comparison of good and poor swimmers (Atlantic Salmon parr) after a maximum swimming effort

Project description:Swimming performance correlates with fitness of Atlantic salmon in the wild and farm conditions. Variation of this trait in salmon populations is high. The study assessed relationship between two parameters of swimming performance: the ability to cope with moderate and maximum swimming efforts.

2021-04-23 | GSE173119 | GEO

Transcription profiling by array of salmon parr brain after exposure to the carbamazepine

Project description:Concerns have arisen recently over the possible environmental effects of human pharmaceuticals. Although acute toxicities are low, the continuous discharge of pharmaceuticals into the aquatic environment, coupled with the fact that such compounds are selected for use on the basis of a strong pharmacological effect, means that sublethal effects on non-target organisms need to be seriously considered. The juvenile stages of Atlantic salmon are present in many northern European rivers which are also used for the discharge of domestic wastewaters likely to contain pharmaceuticals. One year old salmon parr were exposed to an environmentally relevant concentration (5µg·/ L) of the antidepressant drug carbamazepine for five days and changes of mRNA expression in brain tissues were investigated by means of a custom 17k Atlantic salmon cDNA microarray. The TRAITS 17K Atlantic salmon cDNA microarray was employed. A dual-labelled experimental design was employed for the microarray hybridisations. Each experimental cDNA sample (Cy3 labeled) was competitively hybridised against a common pooled-reference sample (Cy5 labeled). The entire experiment comprised 10 hybridisations - 2 states (CBZ exposed / unexposed) × 1 time-point ( at 5 days) × 5 biological replicates (males only). Hybridisations were undertaken concurrently.

2011-08-20 | E-TABM-1065 | biostudies-arrayexpress

Hindgut microbiomes in Atlantic salmon parr

Project description:Changes in rearing water microbiomes in RAS induced by membrane filtration alters the hindgut microbiomes of Atlantic salmon (Salmo salar) parr

| PRJEB48548 | ENA

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