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LncRNA-seq analysis CARM1 knockdown effect on MDA-MB-231 cells

ABSTRACT: Previous studies reported that NEAT1 and its partner p54nrb are necessary for CARM1 to associate with paraspeckles and H3R26 methylation. There are also reports that CARM1 is essential for LincGET's role in guiding the ICM lineage in mouse embryos. We speculate that there may be a connection between CARM1 and lncRNA. In order to verify our guess, we conducted lncRNA-seq experiments against CARM1 control or siRNA in MDA-MB-231 cells to explore the lncRNA that may be regulated downstream of CARM1. Here, we showed that CARM1 and HIF1A co-upregulates the expression of MALAT1 in hypoxia pathway to promote carcinogenesis and metastasis in triple-negative breast cancer cells. These results indicate that there is a connection among CARM1, HIF1A and MALAT1 in triple-negative breast cancer.

ORGANISM(S): Homo sapiens

PROVIDER: GSE168121 | GEO | 2024/03/03

REPOSITORIES: GEO

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RNA-seq analysis CARM1 knockdown effect on MDA-MB-231 cells

Project description:Coactivator associated arginine methyltransferase 1 has been reported to play paramount roles in estrogen receptor alpha (ERα)-positive breast cancer. It promotes breast cancer development and metastasis. However, little research about CARM1 has been focused on triple-negative breast cancer (TNBC). Here, we report that CARM1 promotes proliferation, epithelial-mesenchymal transition (EMT) and stemness in TNBC. CARM1 transactivates Hypoxia-inducible factor 1 subunit alpha (HIF1A) and is physically associated with it. They function together in a concerted complex to regulate downstream targets and promote the carcinogenesis and metastasis of TNBC.

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2017-07-03 | PXD005871 | Pride

Gene expression profiles of CARM1 overexpression in MCF7

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2011-06-01 | E-GEOD-26259 | biostudies-arrayexpress

Global tibialis anterior skeletal muscle transcript data from male Carm1 skm-/- (HSAcre Tg/0 x Floxed Carm1) and Carm1 skm+/+ mice (HSAcre 0/0 x Floxed Carm1) on a chow diet.

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Transcription profiling of mouse embryonic fibroblasts carm1-/- completemented with CARM1 wt were left untreated or were stimulated for 3hours with 10ng/ml mTNFa

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2009-09-01 | E-MEXP-2020 | biostudies-arrayexpress

Gene expression profiles of CARM1 overexpression in MCF7

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Expression profiling of murine neonatal lung tissue from Carm1 knockouts and wild type controls

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2010-07-07 | E-GEOD-21606 | biostudies-arrayexpress

Gene expression profiles of MCF7 with CARM1 knocked down

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2018-09-05 | GSE72848 | GEO

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