Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Transcriptome differences between immortalized brown preadipocytes treated with different paracrine FGFs

ABSTRACT: We analyzed coding transcript abundance in primary, immortalized brown preadipocytes from murine, interscapular brown adipose tissue.

ORGANISM(S): Mus musculus

PROVIDER: GSE168122 | GEO | 2021/03/04

REPOSITORIES: GEO

ACCESS DATA

Json Xml

Similar Datasets

Transcriptome differences between primary brown and white preadipocytes treated with Fgf8b or control

Project description:We analyzed coding transcript abundance in primary brown and white preadipocytes from murine, interscapular brown adipose tissue or inguinal white adipose tissue, respectively.

2021-03-04 | GSE168124 | GEO

Transcriptome differences between immortalized brown preadipocytes treated with different paracrine FGFs

Project description:Transcriptome differences between immortalized brown preadipocytes treated with different paracrine FGFs

| PRJNA706174 | ENA

Transcriptomics of FGF6-stimulated mouse brown preadipocytes

Project description:Uncoupling protein-1 (UCP1) plays a central role in energy dissipation in brown adipose tissue (BAT). Using high-throughput library screening of secreted peptides, we identified two fibroblast growth factors (FGF), FGF6 and FGF9, as novel and potent inducers of UCP1 expression in adipocytes and preadipocytes. Here we show the transcriptome of FGF6-stimulated mouse brown preadipocytes.

2020-02-07 | GSE144061 | GEO

Transcriptome differences between primary brown adipocytes of wildtype and UCP1-KO mice

Project description:We analyzed coding transcript abundance in fully differentiated, primary brown adipocytes from murine, interscapular brown adipose tissue of wildtype and UCP1-KO mice.

2018-09-13 | GSE119873 | GEO

Transcriptome differences between brown adipose tissues of wildtype and UCP1-KO mice

Project description:We analyzed coding transcript abundance in murine, interscapular brown adipose tissue from wildtype and UCP1-KO mice.

2018-11-06 | GSE122133 | GEO

Analysis of the differences in gene expression between wild type and Gpr120 knockout brown adipose tissue

Project description:The goals were to investigate differences in gene expression between wild type and Gpr120 knockout mouse interscapular brown adipose tissue

2018-01-18 | GSE97145 | GEO

Brown and white adipocyte differentiation

Project description:Attainment of a brown adipocyte cell phenotype in white adipocytes, with their abundant mitochondria and increased energy expenditure potential, is a legitimate strategy for combating obesity. The unique transcriptional regulators of the primary brown adipocyte phenotype are unknown, limiting our ability to promote brown adipogenesis over white. In the present work, we used microarray analysis strategies to study primary preadipocytes, and we made the striking discovery that brown preadipocytes demonstrate a myogenic transcriptional signature, whereas both brown and white primary preadipocytes demonstrate signatures distinct from those found in immortalized adipogenic models. We found a plausible SIRT1-related transcriptional signature during brown adipocyte differentiation that may contribute to silencing the myogenic signature. In contrast to brown preadipocytes or skeletal muscle cells, white preadipocytes express Tcf21, a transcription factor that has been shown to suppress myogenesis and nuclear receptor activity. In addition, we identified a number of developmental genes that are differentially expressed between brown and white preadipocytes and that have recently been implicated in human obesity. The interlinkage between the myocyte and the brown preadipocyte confirms the distinct origin for brown versus white adipose tissue and also represents a plausible explanation as to why brown adipocytes ultimately specialize in lipid catabolism rather than storage, much like oxidative skeletal muscle tissue. Keywords: In vitro differentiation

2007-05-31 | GSE7032 | GEO

Identify proteins secreted from mouse brown adipocytes

Project description:Immortalized mouse brown preadipocytes were differentiated. Conditioned medium was collected and concentrated. Proteins present in the conditioned medium were analyzed by LC-MS/MS

2022-11-11 | PXD038041 | Pride

Gene-Expression Profiles in IRS Knockout Brown Preadipocytes

Project description:Insulin and IGF-1 promote adipocyte differentiation via complex and overlapping signalling networks. Here we used microarray analysis of brown preadipocytes derived from wild-type and insulin receptor substrate (IRS) knockout (KO) animals, which exhibited progressively impaired differentiation, to define the set of genes that predict adipogenic potential in these cells. 374 genes/ESTs were identified whose expression in preadipocytes correlated with their ultimate ability to differentiate. Many of these genes were related to early adipogenic events, including genes involved in extracellular matrix, cytoskeletal organization, growth arrest, post-mitotic clonal expansion, and inhibitors of adipogenesis, including preadipocyte factor-1 and multiple members of the Wnt-signalling pathway. Reconstitution of IRS-1 KO cells with IRS-1 reversed these changes and restored the ability to differentiate. Several of these genes showed concordant changes in brown adipose tissue in vivo. Necdin was markedly increased in IRS-1 KO cells that could not differentiate, and knockdown of necdin restored brown adipogenesis with down-regulation of Pref-1 and Wnt10a expression. We demonstrated a necdin-E2F4 interaction repressing PPARg transcription. IRS proteins regulated necdin via a CREB dependent pathway, defining a signalling network involved in brown preadipocyte determination. Keywords = brown fat Keywords = preadipocyte Keywords = adipogenesis Keywords = mouse Keywords: parallel sample

2005-06-01 | GSE2556 | GEO

ChIP-Seq interscapular brown adipose tissue Prdm16

Project description:We report the binding targets of Prdm16 in interscapular brown adipose tissue

2018-08-26 | GSE103434 | GEO