High-fidelity Cas13 variants for targeted RNA degradation with minimal collateral effects

ABSTRACT: We found that several variants with 2-4 mutations on Higher Eukaryotes and Prokaryotes Nucleotide-binding (HEPN) domains showed undiminished on-target activity but markedly reduced collateral effects. Furthermore, transcriptome-wide off-target effects and cell growth arrest induced by wild-type Cas13d were found to be absent for a Cas13d variant. Thus, high-fidelity Cas13 variants with minimal collateral effects are now available for the targeted degradation of RNAs in basic research and therapeutic applications.

ORGANISM(S): Homo sapiens

PROVIDER: GSE168246 | GEO | 2022/06/28

REPOSITORIES: GEO