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YTHDC1-Hyper tribe uncover YTHDC1 binding sites in human leukemia cells

ABSTRACT: The goal of this study is to identify YTHDC1 binding targets in MOLM13 cells(AML).

ORGANISM(S): Homo sapiens

PROVIDER: GSE168568 | GEO | 2021/05/24

REPOSITORIES: GEO

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YTHDC1-Hyper tribe uncover YTHDC1 binding sites in human leukemia cells

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Project description:The goal of this study is to identify differential expressed genes upon YTHDC1 depletion in MOLM13 cells(AML).

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A Critical Role of Nuclear m6A Reader YTHDC1 in Leukemogenesis by Regulating MCM Complex-Mediated DNA Replication

Project description:PLKO.1 lentiviral vectors contained scramble shRNA, YTHDC1 shRNA 1# and YTHDC1 shRNA 2# were transfected into 293T cells together with package plasmids pMDG.2 and Δ8.91. The supernatant medium contained virus was collected and subjected to infect MOLM-13 cells by spinoculation, MOLM-13 cell is an AML cell line. The positive cells were collected 3 days after viral infection and subjected to RNA-seq.

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PAR-CLIP analysis of YTHDC1, SRSF1, SRSF3, SRSF7, SRSF9 and SRSF10 in human HeLa cells

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