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Spear-ATAC: Pooled, droplet-based, single-cell chromatin accessibility CRISPR screens

ABSTRACT: Spear-ATAC is a modified droplet-based single-cell ATAC-seq (scATAC-seq) protocol that enables simultaneous read-out of chromatin accessibility profiles and integrated sgRNA spacer sequences from thousands of individual cells at once. Spear-ATAC profiling of 104,592 cells representing 414 sgRNA knock-down populations across three experiments revealed the temporal dynamics of epigenetic responses to regulatory perturbations in cancer cells and the associations between transcription factor binding profiles, demonstrating a high-throughput method for perturbing and evaluating dynamic single-cell epigenetic states.

ORGANISM(S): Homo sapiens

PROVIDER: GSE168851 | GEO | 2021/03/14

REPOSITORIES: GEO

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