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The impact of late gestation circadian disruption, by two weeks of exposure to chronic light dark phase from 5 weeks before expected calving to 3 weeks expected calving, on dairy cattle hepatic and mammary transcriptomes.

ABSTRACT: Our previous studies found circadian disruption increased insulin resistance and decreased mammary development in late pregnant cows. The effect of circadian disruption on liver and mammary transcriptomes was measured to understand how it impacted hepatic function and mammary development. At 35 d before expected calving (BEC) multiparous Holstein cows were assigned to either control (CON) or phase-shifted treatments (PS). CON was exposed to 16 h light to 8 h of dark, and PS treatment was exposed to 16 h light to 8 h dark, but the phase of the light-dark cycle was shifted 6 h every 3 d. At 21 d BEC, liver and mammary were biopsied. RNA was isolated (n=6 CON and n=6 PS per tissue), libraries prepared and sequenced using paired end reads. Reads mapping to bovine genome averaged 27 M ± 2 M, and aligned to 14,222 protein coding genes in liver and 15,480 in mammary analysis.

ORGANISM(S): Bos taurus

PROVIDER: GSE168914 | GEO | 2021/03/16

REPOSITORIES: GEO

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