Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Transcriptomic profiling of cortical bone from hindlimb unloaded mice

ABSTRACT: The goal of this study was to perform RNA-sequencing of cortical bone isolated from 11-week-old female hindlimb unloaded and control mice. Mice were subjected to hindlimb unloading for 7 days, followed by RNA isolation of cortical bone. Cortical transcriptomic profiles were assessed with RNAseq.

ORGANISM(S): Mus musculus

PROVIDER: GSE169292 | GEO | 2021/03/23

REPOSITORIES: GEO

ACCESS DATA

Json Xml

Similar Datasets

Transcriptomic profiling of cortical bone from hindlimb unloaded mice

Project description:Transcriptomic profiling of cortical bone from hindlimb unloaded mice

| PRJNA716188 | ENA

Transcriptomic profiles of liver tissue from hindlimb unloaded mice treated with 4-phenylbutyrate

Project description:Bulk transcriptomic profiles of liver tissues from male, 18 weeks old c57Bl/6j mice, including ground-based controls, hindlimb-unloaded mice treated with vehicle, and hindlimb unloaded mice treated wit 4-phenylbutyrate (4-PBA)

2024-02-11 | GSE196560 | GEO

RNA-sequencing of skeletal stem cells (SSC) and mesenchymal stem cells (MSC) from hindlimb unloaded and reloaded mice

Project description:Disuse osteoporosis (DO) occurs in response to mechanical unloading of the skeleton and results in structural changes that compromise bone strength leading to increased fracture risk. Although skeletal stem cells (SSCs) are the main source of osteoblasts and adipocytes in adult bone marrow, whether DO impacts SSC function has not been established. Here, three-month-old male C57BL/6 mice were subjected to a hindlimb unloading (HU) model of disuse osteoporosis for 8 or 14 weeks by tail suspension. Control mice (ambulatory or AMB) were allowed normal use of their hindlimbs. An additional cohort underwent an 8-week recovery period after 8-weeks of unloading. Bone marrow-derived, LepR+ skeletal stem cells (SSCs) were isolated by FACS (n=3 or 4) and pooled prior to RNA isolation. Where indicated, cultured, immuno-depleted, bone marrow-derived mesenchymal stem cells (MSCs) were also collected (n=3) and pooled prior to RNA isolation. Generated libraries were subjected to NGS RNA-seq analysis on the Illumina NextSeq 500. These data delineate unique physiological adaptations to prolonged unloading, demonstrate that DO results in increased leptin expression and sympathetic signaling within the BM niche.

2021-12-01 | GSE156174 | GEO

Transcriptomic profiles of testis from hindlimb unloaded mice treated with 4-phenylbutyrate

Project description:Transcriptomic profiles of Testis of hindlimb unloaded mice treated with vehicle or 4-phenylbutyrate (4-PBA)

2024-05-01 | GSE205126 | GEO

A comparison of bone microarchitectural and transcriptomic changes in murine long bones in response to hindlimb unloading and aging

Project description:Age and disuse-related bone loss both result in decreases in bone mineral density, cortical thickness, and trabecular thickness and connectivity. Disuse induces physiological changes in bone similar to those seen with aging. Animal studies used 6, and 22-month-old C57BL/6J male and female mice that were hindlimb unloaded (HLU) for 3 weeks. Ingenuity pathway analysis (IPA) included 4-month-old male mice unloaded for 3 weeks and a publicly available dataset (GLDS647) from 3-month female C57BL/6N mice unloaded for 7 days. There were age- and sex-dependent changes in bone structure and mechanical properties in response to HLU. The cortical bone and epiphyseal trabecular bone compartments were most affected. There were greater changes in bone mechanical properties due to age in males, and HLU in females. RNA extracted from whole-bone marrow-flushed tibiae was sequenced and analyzed. Gene ontology analysis demonstrated that mitochondrial function was downregulated after HLU whereas cell-cycle transition was downregulated with aging. IPA was used to identify the leading canonical pathways and upstream regulators in each HLU age group. IPA identified “Senescence Pathway” as the third leading canonical pathway enriched in mice exposed to HLU. HLU induced activation of the senescence pathway in 3 month and 4-month-old mice, inhibited it in 6-month-old mice and did not change it in 22 month old mice. In conclusion, we demonstrate that hindlimb unloading initiates changes in bone microarchitecture and gene expression characteristic of aging, but with distinct differences.

2023-11-27 | GSE235942 | GEO

Analysis of skeletal muscle gene expression upon mouse hindlimb unloading and reloading

Project description:To investigate the molecular mechanisms governing the transition of skeletal muscle from atrophy to compensatory regeneration and hypertrophy, we employed a mouse model involving hindlimb unloading and subsequent reloading, conducting a comprehensive analysis of global gene expression using RNA-sequencing (RNA-seq). Gastrocnemius muscle samples were obtained from three groups: control mice, mice subjected to 10 days of hindlimb unloading-induced muscle atrophy, and mice reintroduced to normal cage activity for 1 day following the unloading period (reloading).

2023-07-16 | GSE237099 | GEO

Transcriptomic profiles of gastrocnemius muscles from hindlimb unloaded mice treated with 4-phenylbutyrate

Project description:Transcriptomic profiles of gastrocnemius muscles from 18 weeks old, male, hind muscles unloaded mice treated with vehicle or4-phenylbutyrate (4-PBA)

2022-07-20 | GSE189455 | GEO

Hindlimb unloading alters wound healing in ligaments

Project description:A recent physiological study established that hindlimb unloading of rats at 3 and 7 weeks inhibits healing of injured ligaments, resulting in a badly aligned, discontinuous collagen matrix. Using tissue from these rats, we focused on the 3-week time point employing microarray analysis to identify what cellular processes or lack of processes could account for these observed deficiencies. We used the Afyymetrix RG_U34A GeneChip and performed image analysis with Microarray Suite 5.0. For normalization we used the MAS global normalization protocol with a default target mean signal of 500. Gene expression in medial collateral ligament tissue under 4 different treatment conditions was measured: loaded control, loaded wound healing, unloaded control, and unloaded wound healing. From our results, it appears that unloaded tissue lags behind loaded tissue in its progression through the healing process and at 3 weeks is still engaged in the proliferative phase, whereas loaded tissue is actively remodeling its collagen matrix. Summary tables below: Affymetrix MAS comparative analysis data for the 1778 genes qualified for further analysis as a result of detection call values. Keywords: other

2004-03-10 | GSE1105 | GEO

Transcription profiling of rat to study the effect of hindlimb unloading on healing of medial collateral ligaments 3 weeks after injury

Project description:A recent physiological study established that hindlimb unloading of rats at 3 and 7 weeks inhibits healing of injured ligaments, resulting in a badly aligned, discontinuous collagen matrix. Using tissue from these rats, we focused on the 3-week time point employing microarray analysis to identify what cellular processes or lack of processes could account for these observed deficiencies. We used the Affymetrix RG_U34A GeneChip and performed image analysis with Microarray Suite 5.0. For normalization we used the MAS global normalization protocol with a default target mean signal of 500. Gene expression in medial collateral ligament tissue under 4 different treatment conditions was measured: loaded control, loaded wound healing, unloaded control, and unloaded wound healing. From our results, it appears that unloaded tissue lags behind loaded tissue in its progression through the healing process and at 3 weeks is still engaged in the proliferative phase, whereas loaded tissue is actively remodeling its collagen matrix.

2007-10-26 | E-GEOD-1105 | biostudies-arrayexpress

Employing Hindlimb Unloading Model for The Identification of Serum Biomarkers Associated with Cardiovascular and Skeletal Muscle Deconditioning.

Project description:Microgravity and prolonged periods of inactivity cause a variety of diseases, including skeletal muscle mass loss and weakening as well as cardiovascular deconditioning. The primary causes of the inadequate preventative measures for these deconditionings are the lack of biomarkers and unknown underlying mechanisms of cardiovascular and skeletal muscle deconditioning in these conditions. Here, we used a hindlimb unloading (HU) mouse model that replicates astronauts in space and bedridden patients to first evaluate cardiovascular and skeletal muscle performance. Serum samples from these mice were used to identify new biomarkers using metabolomic and proteomic approaches. Three weeks of unloading resulted in alterations in cardiovascular system function in C57/Bl6 mice, as measured by changes in mean arterial pressure and heart weight. Unloading for three weeks also altered skeletal muscle function, resulting in a decrease of grip strength in HU mice, as well as skeletal muscle atrophy, as shown by a drop in muscle mass. A two-week recovery time from the unloading condition partially reversed these alterations, stressing the importance of the recovery process.

2024-01-26 | PXD042753 | Pride

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data