Off-the-shelf proximity labeling

ABSTRACT: In this study we developed a new assay to perform proximity biotinylation in cells without the requirement for genetic manipulation or transfection to fuse a biotin ligase to a protein of interest. We show the specificity by targeting H3K9me3 and performing ChIP-seq for the biotin modification. Targeting IgG was used as a control. By doing so, we identified flywch1 as a new protein binding to H3K9me3 regions, which we validated using ChIP-seq (IgG ChIP was used as a control)

ORGANISM(S): Homo sapiens

PROVIDER: GSE169317 | GEO | 2021/07/09

REPOSITORIES: GEO