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PGE2 supplementation of oocyte culture media improves the developmental and cryotolerance performance of bovine blastocysts derived from a serum-free in vitro production system, mirroring the inner cell mass transcriptome

ABSTRACT: We focused our investigation of an embryo's response to different in vitro environments on changes to the transcriptomic profile of the inner cell mass (ICM), i.e. that part of the blastocyst giving rise to the epiblast and then to all future intraembryonic tissues. We used RNA-sequencing analysis to examine the gene expression of ICM derived under the different IVP conditions. The study evaluated the effects of a serum-free (SF) IVP system including defined oocyte culture media supplemented or not with PGE2, on gene expression of the ICM when compared with standard serum-containing (SC) IVP.

ORGANISM(S): Bos taurus

PROVIDER: GSE169593 | GEO | 2021/04/10

REPOSITORIES: GEO

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