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Genetic determinants of endophytism in the Arabidopsis root mycobiome

ABSTRACT: Roots of Arabidopsis thaliana do not engage in symbiotic association with mycorrhizal fungi but host taxonomically diverse fungal communities that influence health and disease states. We sequenced the genomes of 41 isolates representative of the A. thaliana root mycobiota for comparative analysis with 79 other plant-associated fungi. We report that root mycobiota members evolved from ancestors having diverse lifestyles and retained diverse repertoires of plant cell wall-degrading enzymes (PCWDEs) and effector-like small secreted proteins. We identified a set of 84 gene families predicting best endophytism, including families encoding PCWDEs acting on xylan (GH10) and cellulose (AA9). These genes also belong to a core transcriptional response induced by phylogenetically-distant mycobiota members in A. thaliana roots. Recolonization experiments with individual fungi indicated that strains with detrimental effects in mono-association with the host not only colonize roots more aggressively than those with beneficial activities but also dominate in natural root samples. We identified and validated the pectin degrading enzyme family PL1_7 as a key component linking aggressiveness of endophytic colonization to plant health.

ORGANISM(S): Paraphoma chrysanthemicola Arabidopsis thaliana Chaetomium sp. MPI-CAGE-AT-0009 Phaeosphaeria sp. Macrophomina phaseolina Phaeosphaeria sp. MPI-PUGE-AT-0046c Halenospora varia Truncatella angustata Chaetomium sp.

PROVIDER: GSE169629 | GEO | 2021/11/03

REPOSITORIES: GEO

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2012-03-27 | E-GEOD-34617 | biostudies-arrayexpress

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2011-10-29 | GSE32208 | GEO

Gene expression data from arbuscular mycorrhizal and phosphate-treated Medicago truncatula roots.

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A roadmap of cell-type specific gene expression during early stages of the arbuscular mycorrhiza symbiosis

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