Transcriptomics,Genomics

Dataset Information

32

Induction of pluripotent stem cells from human third molar mesenchymal stromal cells


ABSTRACT: The expression of four transcription factors (OCT3/4, SOX2, KLF4, and c-MYC) can reprogram mouse as well as human somatic cells to induced pluripotent stem (iPS) cells. Expression of the c-MYC, also known as an oncogene, might induce carcinogenesis and thus, iPS cells produced with the use of c-MYC transduction cannot be used for human therapeutic applications. Furthermore, reprogramming efficiency was significantly reduced in the absence of c-MYC transduction. Here, we generated iPS cells from mesenchymal stromal cells (MSCs) derived from human third molars (wisdom teeth) by retroviral transduction of OCT3/4, SOX2, and KLF4 without c-MYC. Interestingly, clonally expanded MSCs, named 10F-15, could be used for iPS cell generation with 100-fold higher efficiency compared to that of other clonally expanded MSCs and human dermal fibroblasts. These iPS cells resembled human embryonic stem (ES) cells in many aspects, including morphology, ES markers expression, global gene expression, epigenetic states, and the ability to differentiate into the three germ layers in vitro and in vivo. Because human third molars are discarded as clinical waste, our data indicate that MSCs isolated from human third molars are a valuable cell source for the generation of iPS cells. Overall design: Three types of cells were used for 1-channel expression analysis: (a) human dermal fibroblasts (1 array) (b) human third molar cells (2 different cell lines, 1 array each) (c) virus-carrying human induced pluripotent stem cells (4 different cell lines, 1 array each)

INSTRUMENT(S): [HG-U133_Plus_2] Affymetrix Human Genome U133 Plus 2.0 Array

SUBMITTER: Hajime Ohgushi  

PROVIDER: GSE16963 | GEO | 2010-06-26

SECONDARY ACCESSION(S): PRJNA117729

REPOSITORIES: GEO

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Publications

Induction of pluripotent stem cells from human third molar mesenchymal stromal cells.

Oda Yasuaki Y   Yoshimura Yasuhide Y   Ohnishi Hiroe H   Tadokoro Mika M   Katsube Yoshihiro Y   Sasao Mari M   Kubo Yoko Y   Hattori Koji K   Saito Shigeru S   Horimoto Katsuhisa K   Yuba Shunsuke S   Ohgushi Hajime H  

The Journal of biological chemistry 20100701 38


The expression of four transcription factors (OCT3/4, SOX2, KLF4, and MYC) can reprogram mouse as well as human somatic cells to induced pluripotent stem (iPS) cells. We generated iPS cells from mesenchymal stromal cells (MSCs) derived from human third molars (wisdom teeth) by retroviral transduction of OCT3/4, SOX2, and KLF4 without MYC, which is considered as oncogene. Interestingly, some of the clonally expanded MSCs could be used for iPS cell generation with 30-100-fold higher efficiency whe  ...[more]

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