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Low molecular weight polysialic acid prevents lipopolysaccharide-induced inflammatory dopaminergic neurodegeneration in humanized SIGLEC11 transgenic mice

ABSTRACT: Here, we investigated the effect of low molecular weight polysialic acid (polySia avDP20) on inflammatory neurodegeneration. SIGLEC11 transgenic and wild type control (WT) mice were treated intraperitoneally daily over four consecutive days with 1 µg/gbw/day LPS in conjunction with 10 μg/gbw polySia avDP20. Repeated LPS injections led to inflammation and neuronal loss in the brains of WT and SIGLEC11 transgenic mice. Transcriptome analysis by RNA-seq of total brain tissue indicated that treatment with polySia avDP20 prevented the LPS-induced upregulation of genes belonging to inflammation, TLR signaling, phagocytosis, and complement-related pathways in SIGLEC11 transgenic but not wild type mice.

ORGANISM(S): Mus musculus

PROVIDER: GSE169637 | GEO | 2021/12/23

REPOSITORIES: GEO

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Genipin inhibits LPS-induced acute systemic inflammation in mice

Project description:Genipin is a natural blue colorant in food industry. Inflammation is correlated with human disorders, and nuclear factor-κB (NF-κB) is the critical molecule involved in inflammation. In this study, the anti-inflammatory effect of genipin on the lipopolysaccharide (LPS)-induced acute systemic inflammation in mice was evaluated by NF-κB bioluminescence-guided transcriptomic analysis. Transgenic mice carrying the NF-κB-driven luciferase genes were administered intraperitoneally with LPS and various amounts of genipin. Bioluminescent imaging showed that genipin significantly suppressed LPS-induced NF-κB-dependent luminescence in vivo. The suppression of LPS-induced acute inflammation by genipin was further evidenced by the reductions of cytokine levels in sera and organs. Microarray analysis of these organs showed that the transcripts of 79 genes were differentially expressed in both LPS and LPS/genipin groups, and one third of these genes belonged to chemokine ligand, chemokine receptor, and interferon (IFN)-induced protein genes. Moreover, network analysis showed that NF-κB played a critical role in the regulation of genipin-affected gene expression. In conclusion, we newly identified that genipin exhibited anti-inflammatory effects in a model of LPSinduced acute systemic inflammation via downregulation of chemokine ligand, chemokine receptor, and IFN-induced protein productions. A total of 25 transgenic mice (female, 6 to 8 weeks old) were randomly divided into five groups of five mice: (1) mock, no treatment; (2) LPS (4 mg/kg), (3) LPS plus genipin (1 mg/kg), (4) LPS plus genipin (10 mg/kg), and (5) LPS plus genipin (100 mg/kg). Mice were challenged intraperitoneally with LPS and then with genipin 10 min later. Four hours later, mice were imaged for the luciferase activity, and subsequently sacrificed for ex vivo imaging, RNA extraction, and immunohistochemical staining.

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Low molecular weight polysialic acid prevents lipopolysaccharide-induced inflammatory dopaminergic neurodegeneration in humanized SIGLEC11 transgenic mice

Project description:Low molecular weight polysialic acid prevents lipopolysaccharide-induced inflammatory dopaminergic neurodegeneration in humanized SIGLEC11 transgenic mice

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Genipin inhibits LPS-induced acute systemic inflammation in mice

2012-02-25 | GSE35934 | GEO

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2020-11-30 | GSE140685 | GEO

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The regulatory roles of Ezh2 in response to lipopolysaccharide (LPS) in macrophages and mice with conditional Ezh2 deletion with LysM-Cre system

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2023-07-20 | PXD041265 | Pride

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