Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

A genome-wide comparison of gene expression patterns in CD4+ T cells under different T cell activating treatments

ABSTRACT: In the literature, several conditions are described that lead to T cell activation. Within this experiment we compared the influence of various T cell activating conditions on the gene expression pattern of CD4+ T cells. T cell stimulation via anti-CD3 was compared to anti-CD3/anti-CD28 as well as to anti-CD3 and IL-2 stimulation.

ORGANISM(S): Homo sapiens

PROVIDER: GSE16993 | GEO | 2018/12/31

REPOSITORIES: GEO

ACCESS DATA

Json Xml

Similar Datasets

Stimulation of gluten-specific CD4+ T cell lines with aCD3/aCD28

Project description:Gluten specific CD4 positive T cell upon anti CD3 and anti CD28 stimulation

2021-02-16 | GSE146441 | GEO

MiR-21 function in T-lymphocytes

Project description:T-lymphocyte activation is efficiently mimicked in vitro by treatment with anti CD3 / anti CD28 antibodies. We report miR-21 induction upon CD3/CD28 stimulation of primary T-lymphocytes. In order to assess the function of miR-21 in T-lymphocytes we interfered with miR-21 function by lentiviral transduction of a miR-21 sponge construct. MRNA profile of miR-21 sponge and control transduced T-lymphocytes 48hrs after stimulation.

2014-10-15 | GSE37213 | GEO

Effect of costimulation on microRNAs levels in human naïve CD4 T cells

Project description:microRNA transcriptional profiling of human naïve CD4 T cells comparing cells that saw no activation, only anti-CD3 or anti-CD3 and anti-CD28 to identify microRNAs specifically upregulated by CD28-mediated costimulation. Three conditions experiment. PBS, anti-CD3, anti-CD3 + anti-CD28 all compared to a reference sample. Biological triplicates, independently extracted and activated.

2012-02-25 | E-GEOD-36071 | biostudies-arrayexpress

Differential effects of inhibition of bone morphogenic protein (BMP) signalling on T-cell activation and differentiation

Project description:Dorsomorphin is a small molecule inhibitor of type I bone morphogenic protein receptors (BMPRs). We have found that dorsomorphin affects a wide range of T cell function. In order to obtain the bigger picture of the effects of DM in T cell activation. transcriptomic analysis was performed using mouse primary CD25-CD4+ T cells with either DM (4 μM) or vehicle in the presence or absence of stimulation by anti-CD3 and -CD28 antibodies. Mouse CD4+ T cells were prepared and cultured with or without stimulation by plate bound anti-CD3 mAb and soluble anti-CD28 mAb for 1 hour. Subsequently, 4 μM DM or DMSO was added and cells were incubated for 6 hours. Total RNA was isolated using Qiagen RNeasy mini kits.

2011-12-01 | E-GEOD-27378 | biostudies-arrayexpress

IL-1β induces the rapid secretion of the antimicrobial protein IL-26 from Th17 cells

Project description:We investigated how IL-1β and anti-CD3/CD28 stimulation affected gene expression in IL-1RI+ and IL-1RI- Th17 cells by RNA sequencing.

2019-06-07 | GSE127457 | GEO

Creld1-deficient CD4+ T cells under homeostatic condition and CD3/CD28 stimulation

Project description:Transciptome profiling of sorted naive CD4+ T cells from control or Creld1 conditional KO mice (CD4-Cre) both directly ex vivo and with or without stimulation with anti-CD3/anti-CD28 antibodies to study the impact of Creld1 on T cell biology

2020-09-09 | GSE133863 | GEO

The effect of IFNα on human CD8 T cells_with other concomitant signals

Project description:IFN alpha mediated gene expression pattern. The effect of IFN alpha on human CD8 T cells responding to antigen (signal 1) and costimulatory signals (signal 2) provided by beads coated with anti-CD3 and anti-CD28 mAbs. This analysis examined the effects of IFN alpha on human CD8 T cells responding to antigen (signal 1) and costimulatory signals (signal 2) provided by beads coated with anti-CD3 and anti-CD28 mAbs. Magnetically sorted untouched CD8+CD45R0- T cells from three different donors were unstimulated or stimulated with IFNa2b or with anti-CD3/CD28 Beads alone or along with IFNa2b or IFNa5 for 48 hours. Individual mRNA samples were analyzed using HG-U133A 2.0 array gene chips. Keywords: Gene expression analysis after different stimulation Magnetically sorted untouched CD8+CD45R0-T cells from three different donors (named A, B and C) unstimulated or stimulated with IFN alpha 2b or with anti-CD3/CD28 beads alone or along with IFN alpha 2b or IFN alpha 5 for 48 hours.

2010-07-23 | E-GEOD-17301 | biostudies-arrayexpress

MiR-21 function in T-lymphocytes

2014-10-15 | E-GEOD-37213 | biostudies-arrayexpress

The effect of IFNα on human CD8 T cells_with other concomitant signals

2010-07-23 | GSE17301 | GEO

Expression data from chimeric antigen receptor transduced (CAR) human CD4+ T cells during expansion

Project description:In this data set we include expression data from human CD4+ T cells isolated on day 0, 6, 11 and 24 follow anti-CD3/anti-CD28 magnetic bead stimulation and chimeric antigen receptor transduction.

2015-01-14 | GSE64914 | GEO

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data