MRC1-Dependent Scaling of the Budding Yeast DNA Replication Timing Program

ABSTRACT: Fourteen yeast mutants with an extended S-phase were identified by a novel genome-wide screen. These mutants are associated with the DNA replication machinery, cell-cycle control and dNTP synthesis. We determined the genome-wide DNA replication timing profile of all these mutants as well as wild type, by FACS-sorting G1- and S-phase cells and co-hybridizing their DNA to Agilent genomic tiling arrays, in four repeats each. We find that MRC1 is required for scaling of the DNA replication timing program upon replication perturbation.

ORGANISM(S): Saccharomyces cerevisiae

PROVIDER: GSE17120 | GEO | 2010/03/04

SECONDARY ACCESSION(S): PRJNA119717

REPOSITORIES: GEO