Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Modulation of energy metabolism and epigenetic landscape in rainbow trout fry by a parental low protein/high carbohydrate diet

ABSTRACT: In rainbow trout (Oncorhynchus mykiss), the effect of a paternal and a maternal high carbohydrate/low protein diet was assessed on progeny. To this purpose, two-year old males and females rainbow trout were fed either a control diet or a high carbohydrate/low protein diet for an entire reproductive cycle for females and for 5 months for males. Crossed-fertilizations were carried out in order to obtain 4 groups of offspring. Before the first feeding, whole fry transcriptomes were compared to detect any impact of the parental diet on offspring metabolism.

ORGANISM(S): Oncorhynchus mykiss

PROVIDER: GSE171310 | GEO | 2021/07/08

REPOSITORIES: GEO

ACCESS DATA

Json Xml

Similar Datasets

No adverse effect of a parental low protein/high carbohydrate diet on their offspring, in rainbow trout (liver)

Project description:In rainbow trout (Oncorhynchus mykiss), the effect of a paternal and a maternal "high carbohydrate/low protein" diet was assessed on progeny. To this purpose, two-year old males and females rainbow trout were fed either a control diet or a "high carbohydrate/low protein" diet for an entire reproductive cycle for females and for 5 months for males. Crossed-fertilizations were carried out in order to obtain 4 groups of offspring. Offspring were fed with a commercial diet until they reached a market size. At this stage, hepatic transcriptomes were compared to detect any impact of the parental diet on offspring metabolism.

2021-08-16 | GSE169001 | GEO

No adverse effect of a parental low protein/high carbohydrate diet on their offspring, in rainbow trout (muscle)

Project description:In rainbow trout (Oncorhynchus mykiss), the effect of a paternal and a maternal high carbohydrate-low protein diet was assessed on progeny at long term. To this purpose, two-year old male and female rainbow trout were fed either a control diet or a high carbohydrate/low protein diet for an entire reproductive cycle for females and for 5 months for males. Crossed-fertilizations were carried out in order to obtain 4 groups of offspring. Offspring were fed with a commercial diet until they reached a market size. At this stage, muscle transcriptomes were analysed to detect any effect of the parental diet on offspring metabolism.

2021-08-16 | GSE169002 | GEO

Programming of the hepatic lipid metabolism via a parental high carbohydrate low protein diet in rainbow trout

Project description:In rainbow trout (Oncorhynchus mykiss), the effect of a paternal and a maternal high carbohydrate-low protein diet was assessed on progeny at long term. To this purpose, two-year old male and female rainbow trout were fed either a control diet or a high carbohydrate/low protein diet for an entire reproductive cycle for females and for 5 months for males. Crossed-fertilizations were carried out in order to obtain 4 groups of offspring. Offspring were fed with a commercial diet until they reached a market size and were then challenged with a complete plant-based diet for 3 months. After the challenge, hepatic transcriptomes were analysed to detect any effect of the parental diet on offspring metabolism.

2022-07-31 | GSE184632 | GEO

Detection of new pathways involved in the acceptance and the utilization of plant-based diet in isogenic lines of rainbow trout fry

Project description:We investigate mechanisms behind the response to a plant based diet at short term, by analyzing the transcriptome of 3 isogenic lines of rainbow trout (R23, AB1, A22). These families represent an interesting genetic material to work on as they have similar responses when fed marine based-diet (M diet) but highly different responses when fed a plant-based diet (V diet). Transcriptomic analyses of whole fry revealed 1740, 1834 and 246 probes differentially expressed among the three isogenic lines. Gene ontology enrichment analysis revealed different enriched pathways. Among them, some were related to immunity and perception. Among ontologies related to metabolism only the methionine metabolism pathway was found to be significantly enriched. First, the use of these isogenic lines of rainbow trout led to find new pathways related to plant-based diets utilization which had not been described previously. Secondly, difference in expression of genes related to immunity suggest that this function play an important role but specific effect on immune status was difficult to assess, and further investigations are needed. Finally, results suggested that mechanisms underlying the use of plant-based diets is more linked to feed intake rather than to metabolism. Research on plants components affecting feed intake should be further explored to continue to improve feed formulation and reduce the reliance of aquaculture on marine resources without affecting fish performance.

2018-09-21 | GSE92673 | GEO

Gene expression in Rainbow trout (Oncorhynchus mykiss) fry gonads after a chronic exposure to a range of 17?-ethynylestradiol concentrations

Project description:Sex steroids play a key role in triggering sec differentiation in fish and the use of exogenous hormone treatment leads to partial or complete sex reversal. This phenomenon has attracted attention since the discovery taht even low environmental doses of exogenous steroids can adversely affect gonad morphology (ovotestis development) and induce reproductive failure. Modern genomic-based technologies have enhanced opportunities to find mechanisms of action (MOA) and dentify biomarkers for the toxic action of a compound. The goal of this study are to improve the understanding of feminization in fish by analyzing gene expression patterns in the gonads of rainbow trout fry after a chronic exposure to several doses (0.01, 0.1, 1 and 10 ?g/L) of ethynylestradiol (EE2) and to offer target genes as potential biomarkers of ovotestis development. An all-male population of Rainbow trout fry was exposed during 76 days (from 60 to 136 days post-fertilization (dpf)) to five nominal concentrations of 17?-ethynylestradiol (0-solvent control-, 0.01, 0.1, 1 and 10 ?g EE2/L of water), using 3 tanks per condition. In total, 30 samples were analyzed independantly: 6 samples per concentration tested (two samples per tank, three tanks per concentration), each sample being a pool of 10 pairs of gonads.

2015-06-25 | E-GEOD-58519 | biostudies-arrayexpress

Modulation of energy metabolism and epigenetic landscape in rainbow trout fry by a parental low protein/high carbohydrate diet

Project description:Modulation of energy metabolism and epigenetic landscape in rainbow trout fry by a parental low protein/high carbohydrate diet

| PRJNA719118 | ENA

Maternal Dietary Choline Levels Cause Transcriptome Shift due to Genotype-by-Diet Interaction in Rainbow Trout (Oncorhynchus mykiss)

Project description:The objective of this study was to identify metabolic regulatory mechanisms affected by choline availability in rainbow trout (Oncorhynchus mykiss) broodstock diets associated with increased offspring growth performance. Three customized diets were formulated to have different levels of choline: (a) 0% choline supplementation (Low Choline: 2065 ppm choline), (b) 0.6% choline supplementation (Medium Choline: 5657 ppm choline), and (c) 1.2% choline supplementation (High Choline: 9248 ppm choline). Six all-female rainbow trout families were fed experimental diets beginning 18 months post-hatch until spawning; their offspring were fed a commercial diet. Experimental broodstock diet did not affect overall choline, fatty acid, or amino acid content in the oocytes (p > 0.05), apart from tyrosine (p ≤ 0.05). Offspring body weights from the High and Low Choline diets did not differ from those in the Medium Choline diet (p > 0.05); however, family-by-diet and sire-by-diet interactions on offspring growth were detected (p ≤ 0.05). The High Choline diet did not improve growth performance in the six broodstock families at final harvest (520-days post-hatch, or dph). Numerous genes associated with muscle development and lipid metabolism were identified, including myosin, troponin C, and fatty acid binding proteins, which were associated with key signaling pathways of lipid metabolism, muscle cell development, muscle cell proliferation, and muscle cell differentiation. These findings indicate that supplementing broodstock diets with choline does regulate expression of genes related to growth and nutrient partitioning but does not lead to growth benefits in rainbow trout families selected for disease resistance.

2024-02-09 | GSE243186 | GEO

Does broodstock nutritional history affect the response of progeny to different first-feeding diets? A whole-body transcriptomic study of rainbow trout alevins

Project description:The whole-body transcriptome of trout alevins was characterized to investigate the effects of long-term feeding of rainbow trout broodstock females a diet free of fishmeal (FM) and fish oil (FO) on the metabolic capacities of progeny. Effects were studied before first feeding and after 3 weeks of feeding with diets containing different proportions of marine and plant ingredients. We showed that that maternal diet history did not significantly affect expression of any gene before the first feeding. We found an effect of maternal nutritional history on gene expression in alevins after 3 weeks of feeding. The major differences in the transcriptome of alevins from VEG-fed females compared to those from COM-fed females were: (i) a down-regulation of genes involved in muscle growth/contraction and (ii) a higher level of expression of genes involved in carbohydrate and energy metabolism related to the delay in growth/ development observed with plant-based diets. Our findings also showed an effect of the first-feeding diets, irrespective of maternal nutritional history. Specifically, the introduction of plant ingredients resulted in up-regulation of genes involved in AA/protein and cholesterol metabolism and in differences in the expression of genes related to carbohydrate metabolism.

2016-10-03 | GSE74271 | GEO

Gene expression in Rainbow trout (Oncorhynchus mykiss) fry gonads after a chronic exposure to a range of 17α-ethynylestradiol concentrations

2015-06-25 | GSE58519 | GEO

Oncorhynchus mykiss liver and muscle cells: Small vs. Large neomale offspring

Project description:Transcriptional profiling of rainbow trout liver and muscle cells comparing small fish with large fish within a population of neomale offspring.

2013-11-20 | GSE52507 | GEO

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data