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The crown-root inducible system (CRIS) coupled to RNAseq allows identifying the early molecular events of crown-root initiation in barley (Hordeum vulgare L.)

ABSTRACT: The molecular regulation of root initiation in monocots is still poorly understood. We report the development of the auxin-based crown-root inducible system (CRIS) in barley (Hordeum vulgare L.). Using this system coupled to RNAseq, we investigated the molecular events occurring during the early steps of crown-root (CR) initiation. The sequencing on the Illumina NovaSeq6000 platform yielded over 123 Gb data, representing more than 408 million reads. Using the last released barley annotation (Morex v2 Gene Models, 2019), we found that 427 genes were downregulated by auxin whereas 764 genes were upregulated. This study provides the first analysis of early event of crown-root initiation in cereals. It will serve as comparison with known genes in rice, maize and other cereals, and could be used in metagenomics studies.

ORGANISM(S): Hordeum vulgare

PROVIDER: GSE171320 | GEO | 2025/12/31

REPOSITORIES: GEO

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