Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Gene expression profiles of cap mesenchyme and renal vesicle isolated between P0-P4 from Crym-EGFP neonatal transgenic mice using FACS. (GUDMAP Series ID: 28)

ABSTRACT: The long term objective is to create an encyclopedia of the expression levels of all genes in multiple components of the developing kidney. The central thesis is straightforward. The combination of fluorescent activated cell sorting (FACS) plus microarray analysis offers a powerful, efficient and effective method for the creation of a global gene expression atlas of the developing kidney. Microarrays with essentially complete genome coverage can be used to quantitate expression levels of every gene in FACS isolated components of the developing kidney. The ensuing rapid read-out provides an expression atlas that is more sensitive, more economical and more complete than would be possible by in situ hybridizations alone.

ORGANISM(S): Mus musculus

PROVIDER: GSE17139 | GEO | 2010/07/16

SECONDARY ACCESSION(S): PRJNA123459

REPOSITORIES: GEO

ACCESS DATA

Json Xml

Similar Datasets

Gene expression profiles of E11.5 metanephric mesenchyme and Adult Juxtaglomerular Complex cells or renal cortex from the kidneys of Crym-GFP or Ren1c-YFP transgenic mice using FACS or single-cell isolation techniques (GUDMAP Series ID:47).

2012-12-05 | GSE42713 | GEO

Project description:The long term objective is to create an encyclopedia of the expression levels of all genes in multiple components of the developing kidney. The central thesis is straightforward. The combination of fluorescent activated cell sorting (FACS) plus microarray analysis offers a powerful, efficient and effective method for the creation of a global gene expression atlas of the developing kidney. Microarrays with essentially complete genome coverage can be used to quantitate expression levels of every gene in FACS isolated components of the developing kidney. The ensuing rapid read-out provides an expression atlas that is more sensitive, more economical and more complete than would be possible by in situ hybridizations alone. YFP & GFP transgenic lines have been used to isolate several cell types including the metanephric mesenchyme, Juxtaglomerular Complex cells or renal cortex from the kidneys of either E11.5 embryos or adult mice. The various cell types were isolated from the kidney using microdissection and single-cell isolation techniques. RNA was isolated from cells and the gene expression profiles were determined by microarrays.

2012-12-05 | E-GEOD-42713 | biostudies-arrayexpress

Gene expression profiles of E15.5 developing podocytes in the developing kidney isolated from MafB-GFP transgenic mice using FACS on 430 2.0 Array chip. (GUDMAP Series ID: 22)

2008-08-04 | GSE12309 | GEO

Gene expression profiles of E15.5 endothelial cells in the developing kidney isolated from TIE2-GFP transgenic mice using FACS. (GUDMAP Series ID: 21)

2008-06-18 | GSE11232 | GEO

Gene expression profiles of E15.5 cap mesenchyme isolated from Six2 transgenic mice using FACS. (GUDMAP Series ID: 23)

2009-01-16 | GSE12588 | GEO

Gene expression profiles of E15.5 peripheral blastema isolated from Meis1-EGFP transgenic mice using FACS. (GUDMAP Series ID: 17)

2007-06-25 | GSE8232 | GEO

Gene expression profiles of adult renal cortical endothelial cells isolated from TIE2-GFP transgenic mice using FACS. (GUDMAP Series ID: 41)

2010-06-23 | GSE22464 | GEO

Gene expression profiles of E10.5 ureteric trunk cells isolated from Hoxb7-GFP transgenic mice using FACS. (GUDMAP Series ID: 40)