Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

HCMV epigenome during early lytic infection

ABSTRACT: Purpose: to investigate occupancy of Pol II and H3K27Ac on the HCMV and cellular genomes at early times post-infection in a lytic model

ORGANISM(S): Homo sapiens Human betaherpesvirus 5

PROVIDER: GSE171512 | GEO | 2022/12/18

REPOSITORIES: GEO

ACCESS DATA

Json Xml

Similar Datasets

HCMV transcriptome during early lytic infection

Project description:Purpose: to investigate expression of HCMV and cellular RNAs at early times post-infection in a lytic model

2022-12-18 | GSE171520 | GEO

Role of the HCMV immediate early proteins in controlling the HCMV and cellular epigenomes

Project description:Purpose: to investigate the role of the HCMV immediate early proteins in controlling the HCMV and cellular epigneomes during lytic infectioin

2022-12-21 | GSE171518 | GEO

Temporal Dynamics of HCMV Gene Expression in Lytic and Latent Infections

Project description:Primary infection with Human cytomegalovirus (HCMV) results in a persistent lifelong infection due to its ability to establish latent infection. During productive HCMV infection, viral genes are expressed in a coordinated cascade that is characteristic of all herpesviruses and traditionally relies on the dependencies of viral genes on protein synthesis and viral DNA replication. In contrast, the transcriptional landscape associated with HCMV latency is still disputed and poorly understood. Here, we examine viral transcriptomic dynamics during the establishment of both productive and latent HCMV infections. These temporal measurements reveal that viral gene expression dynamics along productive infection and their dependencies on protein synthesis and viral DNA replication, do not fully align. This illustrates that the regulation of herpesvirus genes does not represent a simple sequential transcriptional cascade and surprisingly many viral genes are regulated by multiple independent modules. Using our improved classification of viral gene expression kinetics in conjunction with transcriptome-wide measurements of the effects of a wide array of chromatin modifiers, we unbiasedly show that a defining characteristic of latent cells is the unique repression of immediate early (IE) genes. In particular, we demonstrate that IE1 (a central IE protein) expression is the principal barrier for achieving a full productive cycle. Altogether, our findings provide an unbiased and elaborate definition of HCMV gene expression in lytic and latent infection states.

2022-03-29 | GSE193467 | GEO

HCMV infection of human neural precursor cells: HCMV expression

Project description:HCMV treated and control human primary adult neural precursor cells (isolated from hippocampus) were used at passages 2-4 for infection with HCMV and RNA was harvested at indicated times

2012-07-22 | GSE31141 | GEO

Pol2 and H3K27Ac and H3K27me3 occupancy of the viral and host genomes in HCMV infected Kasumi-3 cells at 24 hpi

Project description:Purpose: to compare H3K27Ac and H3K27me3 in HCMV infected and mock infected Kasumi-3 cells at 24 hours post infection and analyze H3K27Ac and H3K27me3 occupancy of the viral genome in GFP+ infected cells

2021-03-05 | GSE164842 | GEO

Functional and molecular dissection of viral lncRNAs throughout HCMV life cycle [longread]

Project description:Small, compact genomes confer a selective advantage to viruses, yet human cyto-megalovirus (HCMV) expresses the long non-coding RNAs (lncRNAs); RNA1.2, RNA2.7, RNA4.9, and RNA5.0. Little is known about the function of these lncRNAs in the virus life cycle. Here, we dissected the functional and molecular landscape of HCMV lncRNAs. We found that HCMV lncRNAs occupy ~30 % and 50~60 % of to-tal and poly(A)+ viral transcriptome, respectively, throughout virus life cycle. RNA1.2, RNA2.7, and RNA4.9, the three abundantly expressed lncRNAs, appear to be es-sential in all infection states. Among these three lncRNAs, depletion of RNA2.7 and RNA4.9 results in the greatest defect in maintaining latent reservoir and promoting lytic replication, respectively. Moreover, we delineated the global post-transcriptional nature of HCMV lncRNAs by nanopore direct RNA sequencing and interactome analysis. We revealed that the lncRNAs are modified with N⁶-methyladenosine (m6A) and interact with m6A readers in all infection states. In-depth analysis demonstrated that m6A machineries stabilize HCMV lncRNAs, which could account for the over-whelming abundance of viral lncRNAs. Our study lays the groundwork for under-standing the viral lncRNA–mediated regulation of host-virus interaction throughout the HCMV life cycle.

2022-11-16 | GSE217710 | GEO

Functional and molecular dissection of viral lncRNAs throughout HCMV life cycle [shortread]

2022-11-16 | GSE217713 | GEO

Temporal Dynamics of HCMV Gene Expression in Lytic and Latent Infections

Project description:Temporal Dynamics of HCMV Gene Expression in Lytic and Latent Infections

| PRJNA796314 | ENA

HCMV infection of human neural precursor cells: Human neural precursor cell expression

2012-07-22 | GSE30912 | GEO

HCMV infection of human neural precursor cells: Human neural precursor cell expression

Project description:HCMV -treated and control human adult neural precurso cells (NPC) were used to extract RNA for profiling on DNA arrays Primary adult hippocampus-derived neural precursor cells were used at passage # 2-4 for HCMV infection, followed by RNA extraction at indicated times Primary adult neural precursor cells were infected with HCMV strains Towne and TR (O.1MOI) and RNA was extracted at 72 hrs postinfection for expression profiling on both HCMV and Affymetrix DNA arrays

2012-07-21 | E-GEOD-30912 | biostudies-arrayexpress

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data