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Transcriptome analysis of 1-year-old Panax vietnamensis Ha et Grushv.

ABSTRACT: Purpose: Panax vietnamensis Ha et Grushv., which contains various valuable ginsenosides, is an important herbal medicine of Vietnam. However, it is an endangered species listed in Vietnam Red Data Book due to over-harvseting. Investigation about genomic or trancriptomic resources is one of the necessary activities to conserve P. vietnamensis Materials and Methods:. In this study, we sequenced the transcriptomes of 1-year-old P. vietnamensis from leaves and roots using Illumina NovaSeqTM6000 system. Results: A total of 60,254,062 and 64,588,528 reads was obtained and then assembled into 45,495 and 49,133 unigenes for leaves and roots, respectively. More than 60% unigenes from two organs were functional annotated using at least one database among Kyoto Encyclopedia of Genes and Genomes, Pfam, Gene Ontology, NCBI non-redundant Protein, and Evolutionary genealogy of genes. Further, the predominant transcripts of each cDNA library were analyzed for different gene expression identification. In addition, 457 unigenes encoding enzymes involved in triterpenoid saponin biosynthesis via the mavelonate (MVA) and the non-MVA (also named as MEP pathways) were discovered.

ORGANISM(S): Panax vietnamensis

PROVIDER: GSE171608 | GEO | 2021/10/09

REPOSITORIES: GEO

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Project description:Panax ginseng C.A. Meyer is a precious Chinese herbal medicine belonging to the Araliaceae family. Because ginseng is extremely taboo, it seriously restricts the sustainable development of the ginseng industry. The allelopathic effect of triterpenoid saponins is one of the important incentives for continuous cropping disorders. However, there are few studies on the mechanism of allelopathic autotoxicity of triterpenoid ginsenosides. Therefore, this study took ginseng hairy roots as the research object, exogenously added ginsenoside Ro to regulate the growth of hairy roots, and measured the changes in its physiological and saponin content. It was found that ginseng hairy roots grew significantly under Ro-0.5 mg/L stress. Inhibition, the specificity of root tip cell injury was obvious, promoted the accumulation of endogenous hormones IAA and SA, and inhibited the accumulation of ABA and JA. The accumulation of ginsenosides was significantly inhibited (except Rg3) under Ro-0.5 mg/L stress. The mRNA analysis of the Ro-0.5 mg/L group and CK group showed that the differentially expressed genes were mostly concentrated in the hormone signal transduction pathway. The genes ARF7 and EFM were up-regulated, and XTH23 and ZOX1 were down-regulated, which can be used as important candidates for hormone-responsive continuous cropping disorders. Gene. 74 differentially expressed miRNAs were identified based on miRNA sequencing, of which 22 were up-regulated and 52 were down-regulated. The target genes of ptc-miR156k_L+1, mtr-miR156b-5p, gma-miR156a_R+1, and mtr-miR156e were all TRINITY_DN14567_c0_g4, TRINITY_DN14567_c0_g4 is a gene in the plant hormone signal transduction pathway, and the four miRNAs are all negatively correlated with mRNA, indicating that ptc-miR156k_L+1, mtr-miR156b-5p, gma-miR156a_R+1, and mtr-miR156e are very likely involved in ginseng Response to continuous cropping disorders and regulation of ginsenoside synthesis. Our findings will provide a useful platform for unraveling the continuous cropping barrier of ginseng and new insights into the genetic engineering of plant stress responses.

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Transcriptome analysis of 1-year-old Panax vietnamensis Ha et Grushv.

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