Single cell RNASeq of whole mouse kidneys from Uromodulin/Tamm horsfall protein knock-out mice

ABSTRACT: To elucidate changes in cellular populations in the ischemic acute kidney injury, we performed single cell RNAseq on mouse kidneys following ischemic reperfusion injury and a sham surgery. Mice were subjected to bilateral clamping of the renal pedicule for 30 minutes. After 6-7 hours of reperfusion the kidneys were removed and processed by a MACs cell isolation protocol and dead cell removal (PMID:33448928) , sorted on a 10X Chromium platform version 3 and libraries generated. Sequencing was performed on an Illumina NovaSeq 6000.

ORGANISM(S): Mus musculus

PROVIDER: GSE171639 | GEO | 2021/04/08

REPOSITORIES: GEO