Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Next Generation Sequencing analysis of the global expression profiling in bone marrow derived macrophages response to porcine circovirus type 2.

ABSTRACT: To know the influences of PCV2 infection on macrophage phenotype, we examined the expression profiling of global genes in PCV2 infected-BMDMs by RNA-seq. The results revealed that expression of 369 genes were greater than twofold in PCV2 infected-BMDMs relative to their expression levels in control cells. In addition, the expression of 201 genes were lower than in PCV2-infected macrophages compared to that of control cells . Among these genes, we found that PCV2 infection strikingly induced expression of a subset of M1-associated genes, whereas decreased expression of multiple M2-associated genes, indicating that PCV2 infection likely reprograms the macrophage transcription phenotype.

ORGANISM(S): Mus musculus

PROVIDER: GSE171721 | GEO | 2021/07/20

REPOSITORIES: GEO

ACCESS DATA

Json Xml

Similar Datasets

Genome-wide transcriptional response of primary alveolar macrophages (PAMs) following infection with porcine circovirus type 2

Project description:Porcine alveolar macrophages (PAMs) play impoartant role in innate immunity. Porcine circovirus type 2 (PCV2) has been identified as the causal agent of postweaning multisystemic wasting syndrome, an economically important multifactorial disease of the swine industry worldwide. We used microarrays to study the transcriptome of PAMs infection with PCV2. PAMs were collected by bronchoalveolar lavage from health piglets (free of PCV2, PRRSV, PRV, CSFV, PPV), and PAMs were cultured for 48 hours and inoculated with 5 moi of PCV2.

2011-07-26 | E-GEOD-30918 | biostudies-arrayexpress

Genome-wide transcriptional response of primary alveolar macrophages (PAMs) following infection with porcine circovirus type 2

2011-07-27 | GSE30918 | GEO

Ileal gene expression signatures of piglets after PCV2 infection

Project description:To further study of pcv2-related diseases, we have employed whole genome microarray expression profiling as a discovery platform to identify ileal differentially expressed genes of piglets after pcv2 infection. Infected and uninfected piglets were sampled and analyzed by whole genome microarray expression profiling. The result showed 43603 differencially expressed genes in ileum after PCV2 infection. Expression of 11 genes (IL-1α, IL-1β, IL-6, IFNε, C5, C1QA, CCL4, CCL5, CCL25, CXCL9, CD163) from this signature was quantified in the same RNA samples by real-time PCR, confirming low variability between samples.

2020-10-27 | GSE149144 | GEO

Transcription profiling of pig mediastinal lymph nodes from piglets experimentally infected with porcine circovirus type 2 (PCV2)

Project description:This study aimed to characterize differences in gene expression in piglets inoculated with porcine circovirus type 2 (PCV2), the essential causative agent of postweaning multisystemic wasting syndrome (PMWS). Comparisons between control and PCV2-inoculated pigs were done at five different time points: 1, 2, 5, 8, and 29 days post-inoculation. Experiment Overall Design: Seven-day-old caesarean-derived, colostrum-deprived piglets were distributed into two groups: control (n=8) and inoculated with 105.2 TCID50 of the Burgos PCV2 isolate (n=16). One control and 3 inoculated pigs were necropsied on days 1, 2, 5, and 8 post-infection (p.i.), the remaining pigs (4 of each group) were necropsied on day 29 p.i.

2009-11-15 | E-GEOD-14758 | biostudies-arrayexpress

Changes in cellular microRNA expression induced by porcine circovirus type 2-encoded proteins.

Project description:We employed deep sequencing technology to uncover cellular miRNAs differentially regulated after expression of each of three PCV2-encoded open reading frames (ORFs) in porcine kidney epithelial PK15 cells. Control PK15 vs. PCV2 ORF1, ORF2, ORF3-expressing PK15.

2014-08-08 | E-GEOD-60206 | biostudies-arrayexpress

Expression data from blood samples of piglets experimentally infected with porcine circovirus type 2 (PCV2)

2009-12-31 | GSE14790 | GEO

Expression data from mediastinal lymph nodes of piglets experimentally infected with porcine circovirus type 2 (PCV2)

2009-11-04 | GSE14758 | GEO

Expression data from blood samples of piglets experimentally infected with porcine circovirus type 2 (PCV2)

Project description:This study aimed to characterize differences in gene expression in piglets inoculated with porcine circovirus type 2 (PCV2), the essential causative agent of postweaning multisystemic wasting syndrome (PMWS). Comparisons between control and PCV2-inoculated pigs were done at five different time points: 0, 7, 14, 21 and 29 days post-inoculation. Keywords: time course Seven-day-old caesarean-derived, colostrum-deprived piglets were distributed into two groups: control (n=4) and inoculated with 105.2 TCID50 of the Burgos PCV2 isolate (n=4). Pigs were bled at 0, 7, 14, 21, and 29 days post-inoculation.

2009-12-31 | E-GEOD-14790 | biostudies-arrayexpress

Transcriptome analysis of long non-coding RNAs in Mycobacterium avium complex-infected macrophages

Project description:Mycobacterium avium complex (MAC) is a non-tuberculosis mycobacteria widely distributed throughout environment. Even though the MAC infection is currently increasing in old women and patients with affected immune system, comprehensive analysis of MAC-infected host cell transcriptome, in particular that of long non-coding RNAs (lncRNAs), has scarcely been reported. Using in vitro cultured primary mouse bone marrow-derived macrophages (BMDMs) and CAGE technology, we analyzed the transcriptional and kinetic landscape of macrophage genes, with focusing on lncRNAs, during MAC infection. MAC-infected macrophages induced immune/inflammatory response genes and other genes as similar as M1 activation, consistent with previous reports, although Nos2 and Arg1 revealed distinct expression profiles from M1 activation. We identified 31 up-regulated and 30 down-regulated lncRNA promoters corresponding to 18 and 27 lncRNAs, respectively. The up-regulated lncRNAs were clustered into two groups, early and late up-regulated groups, which were predicted to associate with the immune activation and the immune response to infection, respectively. Further, the Ingenuity IPA analysis predicted canonical pathways and upstream transcription regulators associated with differentially expressed lncRNAs. Several differentially expressed lncRNAs that have been reported elsewhere revealed various expressional change by M1 or M2 pre-activation and the following MAC infection. Finally, we showed that expressional change of lncRNAs in MAC-infected BMDMs is predominantly mediated by Tlr2, but there may be other weakly sensing mechanism for MAC infection. Taken together, we identified differentially expressed lncRNAs in MAC-infected BMDMs, which revealed diverse features implying their distinct role in MAC infection and macrophage polarization.

2024-01-01 | GSE249460 | GEO