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Single cell transcriptional profiles of islet1-derived ECs and the other ECs in the tail of 48 hpf zebrafish embryos

ABSTRACT: We report transcriptional heterogeneity of venous endothelial cells (ECs) in the tail of zebrafish embryos, which consist of HSPC-niche-constituting ECs and caudal vessel (CV)-constituting ECs. To characterize isl1-derived ECs which derive from the endoderm and mainly constitute the HSPC niche in the caudal hematopoietic tissue (CHT), we performed single cell RNA sequencing (scRNA-seq) of isl1-derived ECs and the other ECs separately isolated from the tails of zebrafish embryos. Our analyses revealed that tail venous ECs were split into 5 distinct sub-clusters where isl1-derived ECs and the other ECs were similarly distributed to all venous EC clusters, and further revealed that genes whose expression levels are different between isl1-derived ECs and the other ECs tend to show similar changes across all of the clusters even after their diversification.

ORGANISM(S): Danio rerio

PROVIDER: GSE171822 | GEO | 2022/12/19

REPOSITORIES: GEO

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