Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Dynamic profiling of histone Kcr and Kla during neural differentiation in P19 embryonal carcinoma cells

ABSTRACT: To gain a deeper insight into dynamic changes of histone lysine crotonylation and lactylation during embryonic neurogenesis, ChIP-seq, ATAC-seq and RNA-seq were performed to analyze the genome-wide changes of histone Kcr and Kla in the P19 embryonal carcinoma cells.

ORGANISM(S): Mus musculus

PROVIDER: GSE171832 | GEO | 2022/06/30

REPOSITORIES: GEO

ACCESS DATA

Json Xml

Similar Datasets

Dynamic profiling of histone Kcr and Kla during neural differentiation in vivo

Project description:To gain a deeper insight into dynamic changes of histone lysine crotonylation and lactylation during embryonic neurogenesis, ChIP-seq, ATAC-seq and RNA-seq were performed to analyze the genome-wide changes of histone Kcr and Kla in the developing telencephalon.

2022-06-30 | GSE171088 | GEO

Dynamic profiling of histone Kcr and Kla during neural differentiation in P19 embryonal carcinoma cells

Project description:Dynamic profiling of histone Kcr and Kla during neural differentiation in P19 embryonal carcinoma cells

| PRJNA721095 | ENA

Profiling of H3K9cr and H3K9ac in the P19 embryonal carcinoma cells

Project description:To gain a deeper insight into dynamic changes of H3K9cr and H3K9ac during cell fate commitment, ChIP-seq was performed to analyze the genome-wide changes of H3K9cr and H3K9ac during neuroectodermal differentiation of the P19 embryonal carcinoma cells.

2022-04-17 | GSE200519 | GEO

Identification of 67 histone marks and histone lysine crotonylation as a new type of histone modification

Project description:We report the identification of 67 previously undescribed histone modifications, increasing the current number of known histone marks by about 70%. We further investigated one of the marks, lysine crotonylation (Kcr), confirming that it represents an evolutionarily-conserved histone posttranslational modification. The unique structure and genomic localization of histone Kcr suggest that it is mechanistically and functionally different from histone lysine acetylation (Kac). Specifically, in both human somatic and mouse male germ cell genomes, histone Kcr marks either active promoters or potential enhancers. In male germinal cells immediately following meiosis, Kcr is enriched on sex chromosomes and specifically marks testis-specific genes, including a significant proportion of X-linked genes that escape sex chromosome inactivation in haploid cells. These results therefore dramatically extend the repertoire of histone PTM sites and designate Kcr as a specific mark of active sex chromosome-linked genes in postmeiotic male germ cells. 2 histone marks (pan-lysine acetylation and pan-lysine crotonylation) were studied in 1 human cell type and 2 mouse cell types using ChIP-Seq. Input was sequenced for each cell type as a control. Pan-anti_Kac and pan-anti_Kcr antibodies were custom developed with PTM BioLab, Co., Ltd (Chicago, IL).

2011-10-06 | E-GEOD-32663 | biostudies-arrayexpress

Genome-wide profiling of histone lysine crotonylation reveals positive regulation of transcription in rice

Project description:Histone lysine crotonylation (Kcr) is a newly discovered post-translational modification (PTM) existing in mammalian. To assess relevance in histone Kcr and genome, we performed on genomic localization analysis of histone Kcr by ChIP-seq analysis.

2018-06-01 | GSE108542 | GEO

P19-atRA-kinetics

Project description:P19 embryonal carcinoma cells were treated with 1µM all trans retinoic acid and total RNA was collected at different time points

2013-06-30 | E-MEXP-3669 | biostudies-arrayexpress

Distribution of chromatin features in mouse P19 embryonal carcinoma cells

Project description:Distribution of chromatin features in mouse P19 embryonal carcinoma cells

| PRJNA484121 | ENA

Hypoxia promotes histone H3K9 lactylation to enhance LAMC2 transcription in esophageal squamous cell carcinoma

Project description:Hypoxia promotes tumorigenesis and lactate accumulation in esophageal squamous cell carcinoma (ESCC). Lactate can induce histone lysine lactylation (Kla, a recently-identified histone marks) to regulate transcription. However, the functional consequence of histone Kla under hypoxia in ESCC remains to be explored. Here, we reveal that hypoxia facilitates histone H3K9la to enhance LAMC2 transcription for proliferation of ESCC. We found that global level of Kla was elevated under hypoxia, and thus identified the landscape of histone Kla in ESCC by quantitative proteomics. Furthermore, we show a significant increase of H3K9la level induced by hypoxia. Next, MNseq ChIP-seq and RNA-seq analysis suggest that H3K9la is enriched at the promoter of cell junction genes. Finally, we demonstrate that the histone H3K9la facilitates the expression of LAMC2 for ESCC invasion by in vivo and in vitro experiments. Briefly, our study reveal a vital role of histone Kla triggered by hypoxia in cancer.

2024-05-22 | GSE255352 | GEO

ChIP-seq analysis of porcine alveolar macrophages infected with T. gondii RH

Project description:Toxoplasma gondii (T. gondii) is an obligate intracellular parasite that can infect almost all warm-blooded animals, causing serious public health problems. Lysine crotonylation (Kcr) is a newly discovered posttranslational modification (PTM), which has been proved that is relevant to procreation regulation, active transcription and cell signaling pathway. However, the biological functions of crotonylation have not yet been reported in macrophages infected with T. gondii. In our study, we performed a ChIP-seq analysis of porcine alveolar macrophages infected with T. gondii RH to explore the relationship of histone Kcr with T. gondii infection.

2021-12-31 | GSE167340 | GEO

ChIP-seq and RNA-seq analysis of histone Kcr in the embryonic forebrain and neural stem/progenitor cells.

Project description:To gain a deeper insight into how histone lysine crotonylation regulates embryonic neural stem/progenitor cells (NSPCs) proliferation and differentiation, ChIP-seq and RNA-seq were performed to analyze the genome-wide changes of histone Kcr and transcriptiome changes stimulated with crotonate and MS-275 in E13.5 NSPCs.

2021-06-22 | GSE124540 | GEO

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data