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Transcriptome sequencing of liver tissue in hepatocyte-specific IRF8-deficient mice (IRF8-cKO) mice and wild type (WT) mice that were subjected to warm ischemia/reperfusion(I/R) injury

ABSTRACT: Interferon regulatory factor 8 (IRF8), also known as interferon consensus sequence-binding protein, is an essential lineage-specific transcriptional regulator in the IRF family, controls diverse cellular processes and is has been implicated in innate immunity, immune cell differentiation. IRF8 has been shown to have both oncogenic and tumor suppressor activities, in myeloid cells and nonhematopoietic cells. As a transcription factor, other functions which may contribute to its oncogenic and anti tumor potential are not clear. So it is meaningful to study the role of IRF8 in different diseases. Our recent study demonstrated the protective effects of IRF8 on the liver cancer through the regulation of immune response. In addition, studies have shown that IRF8 has protective effects on ischemia-reperfusion injury of heart and brain and we wondered. However, the role of IRF8 in hepatic ischemia-reperfusion injury has not been reported.

ORGANISM(S): Mus musculus

PROVIDER: GSE171982 | GEO | 2024/04/13

REPOSITORIES: GEO

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Stable overexpression of IRF8 in hepatocellular carcinoma cells hepa1-6 xenograft derived tumors in C57bl/6 mice

Project description:Interferon regulatory factor 8 (IRF8), also known as interferon consensus sequence-binding protein, is an essential lineage-specific transcriptional regulator in the IRF family, controls diverse cellular processes and is has been implicated in innate immunity, immune cell differentiation. IRF8 has been shown to have both oncogenic and tumor suppressor activities, in myeloid cells and nonhematopoietic cells. As a transcription factor, other functions which may contribute to its oncogenic and anti tumor potential are not clear. So it is meaningful to study the role of IRF8 in different diseases. The roles and the underlying mechanisms of intrahepatic IRF8 in Hepatocellular carcinoma have not been reported up to date.

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Expression of IRF8 in gastric epithelial cells confers protective innate immunity against Helicobacter pylori infection

Project description:The transcription factor IRF8 is expressed in many types of blood cells and plays critical roles in cellular differentiation and function. However, the role of IRF8 in nonhematopoietic systems remains poorly understood. In this study, we examined the role of IRF8 in gastric epithelial cells (GECs) and provided evidence that IRF8 is a transcriptional modulator of the gastric mucosa necessary for limiting H. pylori colonization. H. pylori infection significantly upregulated expression of IRF8, which in turn promoted IFN-γ expression by GECs. Mice deficient of IRF8 exhibited increased H. pylori colonization and aborted induction of mucosal IFN-γ. Genome-wide analyses of IFN-γ-treated GECs by ChIP-seq and RNA-seq led to identification of IRF8 target genes with many belonging to the IFN regulated gene family that was previously observed in immune cells. Our results identify the IRF8- IFN-γ circuit as a previously unrecognized gastric innate immune mechanism in defense against infection of H. pylori.

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Ischemic Pre- and Post Conditioning has pronounced effects on Gene Expression Profiles in the Rat Liver after Ischemia/Reperfusion

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2011-09-30 | E-GEOD-24430 | biostudies-arrayexpress

Comparative analysis of brain transcriptomes altered during JEV infection in wild type and IRF8 knockout C57Bl/6 mice

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Ischemic Pre- and Post Conditioning has pronounced effects on Gene Expression Profiles in the Rat Liver after Ischemia/Reperfusion

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A dual cis-regulatory code links IRF8 to constitutive and inducible gene expression in macrophages.

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