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MRNA expression profiles of 223 cancer cell lines including newly established ones

ABSTRACT: Establishment and molecular characterization of 239 peritoneally-metastatic cancer cell lines from 170 patients’ ascites in 11 kinds of cancers mainly consisted of gastric, pancreatic, and ovarian cancer. We performed comparative transcriptome analyses using microarrays between our established cancer cell lines and cell-bank derived cancer cell lines

ORGANISM(S): Homo sapiens

PROVIDER: GSE172159 | GEO | 2023/02/21

REPOSITORIES: GEO

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mRNA expression profiles of newly established 49 gastric cancer cell lines.

Project description:Establishment and molecular characterization of 49 peritoneally-metastatic gastric cancer cell lines from 18 patients’ ascites. We performed comprehensive transcriptome analyses using microarrays of our established gastric cancer cell lines.

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mRNA expression profiles of 7 gastric cancer cell lines and primary cancer cells.

Project description:Molecular characterization of 7 peritoneally-metastatic gastric cancer cell lines and primary cancer cells established from a patients’ ascites. We performed comprehensive transcriptome analyses using microarrays of our established gastric cancer cell lines and primary cancer cells.

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Expression profile of maliganant mesothelioma cell lines

Project description:transcriptional profiling of makignant mesothelioma cell lines comparing control one immortalized mesothelial cell line, MeT-5A, and 2 primary normal mesothelial cultures collected from ascites of non-cancer patients, OV-M1 and GAS-M1

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Innate immune agonist, dsRNA, induces apoptosis in ovarian cancer cells and enhances the potency of cytotoxic chemotherapeutics

Project description:This study aimed at characterizing the effect of the innate immune agonist, dsRNA, on ovarian cancer cell lines and ascites-derived ovarian cancer cells. DsRNA stimulation revealed two sub-populations of ovarian cancer cells, dsRNA sensitive and dsRNA resistant, that could be categorized based upon dsRNA-induced dsRNA receptor expression. Human ovarian cancer cell lines were treated with pI:pC and compared against untreated cells.

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Project description:Direct library preparation+ single-cell DNA-sequencing of (i) patient derived triple negative breast cancer xenograft (ii) primary tumour and ascites ovarian cancer cell lines at tumour recurrence.

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Ascites enriches for ovarian cancer stem-like cells that express membrane GRP78

Project description:Ovarian cancer patients are generally diagnosed at stage III/IV, when ascites is common. The volume of ascites positively correlates with the extent of metastasis and negatively with prognosis. Membrane GRP78, a stress-inducible endoplasmic reticulum chaperone which also appears on the plasma membrane (memGRP78) of aggressive cancers, plays a crucial role in the maintenance of embryonic stem cells. Our present study demonstrates that tumor cells isolated from ascites generated by epithelial ovarian cancer (ID8 cells) bearing mice have increased memGRP78 expression compared to ID8 cells in normal culture. We hypothesize that these ascites associated memGRP78+ cells are cancer stem-like cells (CSC) and memGRP78 is functionally important in CSCs. Supporting this hypothesis, we show that memGRP78+ cells isolated from ascites have increased sphere forming and tumor initiating abilities compared to memGRP78- cells. When the tumor microenvironment is recapitulated by adding ascites fluid to cell culture, ID8 cells express more memGRP78 and increased self-renewing ability compared to those cultured in medium alone. Moreover, compared to their counterparts cultured in normal medium, ID8 cells cultured in ascites, or isolated from ascites, show an increased expression of stem cell markers Sca-1, Snail and SOX9. Importantly, antibodies directed against the carboxy (COOH)-terminal domain of GRP78 significantly reduce the self-renewing ability of murine and human ovarian cancer cells pre-incubated with ascites, associated with a decreased phosphorylation of Akt and GSK3α, and reduced level of the transcriptional factor Snail. Based on this data, we suggest that memGRP78 is a logical therapeutic target for late stage ovarian cancer. Two types of ovarian cancer cells from different organ sites are profiled by gene expression. Parental cells (ID8) and ID8 cells which have metastasized to Ascites (AS).

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2020-12-31 | GSE100627 | GEO

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