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Metabolic capacities of Novosphingobium sp. HR1a in the rhizosphere

ABSTRACT: Rhizoremediation, the biotechnology of the utilization of rhizospheric microorganisms associated with plant roots for the elimination of soil contaminants, is based on the ability of microorganisms to metabolize nutrients from plant root exudates, in order to survive the stressful conditions of the rhizosphere, and thereby, to co-metabolize or even mineralize toxic environmental contaminants. Novosphingobium sp. HR1a is a bacterial strain able to degrade a wide variety of polycyclic aromatic hydrocarbons (PAHs). We have demonstrated that this bacterium is able to grow in vegetated microcosms and to eliminate phenanthrene in the presence of clover faster than in non-vegetated systems, establishing a positive interaction with clover. We have studied the molecular basis of this interaction by phenomic, metabolomic and transcriptomic analyses, demonstrating that the positive interaction between clover and Novosphingobium sp. HR1a is a result of the bacterial utilization of different carbon and nitrogen sources (such as sugars, amino acids and organic acids) released during seedling development, and the capacity of exudates to induce the PAH degradation pathway. These results are pointing out to Novosphingobium sp. HR1a as a promising strain for the bioremediation of PAH-contaminated soils.

ORGANISM(S): Novosphingobium sp. HR1a

PROVIDER: GSE172273 | GEO | 2021/04/19

REPOSITORIES: GEO

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PahT regulates carbon fluxes in Novosphingobium sp. HR1a and influence its survival in soil and rhizosphere.

Project description:In this work we describe the role of paht gene from Novosphingobium sp. HR1a, as a regulator that seems to be involved in the control over the utilization of carbon and sulfur sources mainly involved in the control of pyruvate production and in the acetyl-CoA biosynthesis and PEP recycling from pyruvate.

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Transcriptomic analysis of responses to exudates reveal genes required for rhizosphere competence of the endophyte Azoarcus sp. strain BH72.

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2012-11-30 | E-GEOD-35187 | biostudies-arrayexpress

Transcriptomic analysis of responses to exudates reveal genes required for rhizosphere competence of the endophyte Azoarcus sp. strain BH72.

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