Genomics

Dataset Information

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Human Cells Time of Replication (ToR)


ABSTRACT: Measurment of the ToR by comapring the DNA content of the S phase and the DNA content of the G1 phase, the method is based on the method used in the paper - Replication timing of the human genome by Woodfine et al. that was published in Hum. Mol. Genet. 2004. Briefly, after staining with Propidium Iodide, cells in G1 and S phase of the cell cycle are sorted by FACS sorter (ARIA or Vantage), DNA is then extracted from the fractions and differentially labeled with dUTP-Cy3 or dUTP-Cy5 using direct labeling (Agilent labeling kit). The labeled DNA is cohybridized to the array. Early replicating sequences correspond to the spots with high S/G1 ratio and late replicating sequences correspond to the spots with low S/G1 ratio.

ORGANISM(S): Homo sapiens

PROVIDER: GSE17235 | GEO | 2010/05/25

SECONDARY ACCESSION(S): PRJNA123667

REPOSITORIES: GEO

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