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Next Generation Sequencing Analysis of SRSF1 flox/flox and SRSF1-KO pancreatic islet Transcriptome

ABSTRACT: we report insulin secretion defects in SRSF1 Ins2-Cre mice, in which SRSF1 is ablated in pancreatic β cells.

ORGANISM(S): Mus musculus

PROVIDER: GSE173455 | GEO | 2023/04/17

REPOSITORIES: GEO

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Next-generation sequencing analysis of 7-month-old SRSF2 flox/flox and SRSF2-PKO mice pancreatic islet transcriptome

Project description:We report insulin secretion defects in SRSF2 Ins2-Cre mice, in which SRSF2 is ablated in pancreatic β cells.

2024-04-28 | GSE175969 | GEO

Next Generation Sequencing Analysis of Wild Type and SRSF1-MKO Skeletal Muscle Transcriptomes

Project description:Skeletal muscle specific SRSF1 KO mice were generated by mating SRSF1flox/flox and MyoD-Cre mice, Total RNA from muscle sample were analyzed by Next Generation Sequencing

2018-10-01 | GSE111671 | GEO

Splicing Factor SRSF1 Deficiency in the Liver Triggers NASH-like Pathology via R-Loop Induced DNA Damage and Cell Death

Project description:Regulation of RNA processing contributes profoundly to tissue development and physiology. The serine-arginine-rich splicing factor 1 (SRSF1) is essential for hepatocyte function and survival. Although SRSF1 is mainly known for its many roles in mRNA metabolism, it is also crucial for maintaining genome stability. We show that acute liver damage in the setting of targeted SRSF1 deletion in mice is primarily mediated by the excessive formation of deleterious RNA–DNA hybrids (R-loops), which induce DNA damage. Combining hepatocyte-specific transcriptome, proteome, and RNA binding analyses, we demonstrate that widespread genotoxic stress following SRSF1 depletion results in global inhibition of mRNA transcription and protein synthesis, leading to impaired metabolism and trafficking of lipids. Accumulation of lipids in SRSF1-deficient hepatocytes is quickly followed by necroptotic cell death, inflammation, and fibrosis, resulting in NASH-like liver pathology. This pathogenesis is recapitulated in SRSF1-depleted human liver cancer cells illustrating a conserved and fundamental role for SRSF1 in preserving genome integrity and tissue homeostasis. This data set contains a proteomic comparison of hepatocytes from wild type vs. acute knockout of SRSF1. The acute knockout was generated by injecting 8-week-old SRSF1 fl/fl mice with a viral vector expressing Cre under the control of the liver-specific thyroxine binding globulin (TBG) promoter (AAV8-TBG-iCre). Controls were generated by injecting AAV8-TBG-GFP viral vector. The hepatocytes were isolated 2 weeks post injection.

2023-03-11 | PXD027035 | Pride

Chip-chip from Mouse MEFs cells for H3K36me3, Srsf1, Psip1(A300-847 antibody).

Project description:The p52 isoform of Psip1/Ledgf links histone H3K36 methylation and the regulation of alternative splicing. Chromatin immunoprecipitation (ChIP) of Psip1 together with H3K36me3 and Srsf1 and by ChIP-on-chip analysis demonstrated that H3K36me3, Psip1 and SRSF1 enrichment correlates on the gene bodies Array design includes 2 dye swap replicates for Srsf1 and Psip1-/- samples, and single arrays for PSIP and H3K36me3 samples

2012-01-01 | E-GEOD-31520 | biostudies-arrayexpress

SRSF1 HITS-CLIP

Project description:SRSF1 HITS-CLIP

| PRJNA541847 | ENA

SRSF1 HITS-CLIP in MDA-LM2

Project description:To identify SRSF1 binding sites on RNAs, we performed HITS-CLIP of endogenous SRSF1 in MDA-LM2 cells

2019-12-26 | GSE131745 | GEO

SRSF1 HITS-CLIP

Project description:To identify METTL3 binding sites on RNAs, we performed HITS-CLIP of endogenous SRSF1

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Next Generation Sequencing Analysis of Wild Type (WT) and SRSF1 knockdown (KD) U87MG and U251 cells Transcriptomes

Project description:We generated the SRSF1 knockdown U87MG and U251 cells by infecting with shRNA virus. Then, we extracted RNAs and performed next generation sequencing. By comparing sequencing data from WT and KD samples in each cell type, we profiled the alternative splicing events and gene expression regulated by SRSF1 in GBM.

2020-01-09 | GSE109436 | GEO

SRSF1 role in cellular gene expression and splicing

Project description:SRSF1 is an abundant RNA-binding protein with functions in mRNA splicing, stability, translation and transcription of cellular and viral genes. We analyzed the role that SRSF1 plays in cellular gene transcription and splicing by transfecting HEK293 cells with a SRSF1 expression plasmid and by analyzing the transcriptome of the transiently transfected cells 15 hrs and 48 hrs post transfection. We also explored the role of the SRSF1 domains by transfecting a deletion clone of SRSF1 carrying only the RNA Recognition Motifs 1 and 2 (RRM1,2) but not the Arg-Ser rich (SR) domain.

2019-03-20 | GSE124397 | GEO

Total RNA-seq of siRNA-mediated SRSF1 knockdown

Project description:To identify changes in splicing patterns following SRSF1 knockdown, RNA-sequencing was performed on breast cancer cells transfected with siRNAs targeting SRSF1.

2019-12-26 | GSE130868 | GEO

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