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RNA-Seq of CD8+ T cell subsets during LCMV infection [RNA-seq_D5_JQ1]

ABSTRACT: P14 cells were transferred to naïve mice subsequently infected with LCMV. Mice were treated with JQ1 or vehicle on days 1-4 of infection. On day 5 of infection KLRG1loCD127lo EEC were sorted for RNAseq analysis. In a separate experiment, P14 cells were transduced with shBrd4a or shBrd4b and mixed with shCtrl transduced P14 cells, and then transferred into naïve mice subsequently infected with LCMV. On day 5 post-infection, transduced EEC were sorted for RNAseq.

ORGANISM(S): Mus musculus

PROVIDER: GSE173510 | GEO | 2021/05/21

REPOSITORIES: GEO

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miRNA signatures of antigen specific CD8+ T cells at different stages of immune response to LCMV infection

Project description:miRNAs play an important role in regulating CD8+ T cell response. We used the microarray approach to profile the miRNA signatures of naïve, day 5 effector, day 8 effector, and memory CD8+ T cells. We identified a miRNA signature associated with rapidly proliferating effector CD8+ T cells. CD8+ T cells from P14 TCR transgenic mice were transferred to C57BL6 recipients which were subsequently infected with LCMV Armstrong. Donor P14 CD8+ T cells were sorted on day 5, day 8, or >day 60 post-infection. Naïve P14 CD8+ T cells were sorted directly from naive P14 splenocytes. The total RNA including miRNAs was extracted from sorted samples, labeled, and hybridized to Agilent Mouse miRNA microarray.

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RNA-Seq of CD8+ T cell subsets during LCMV infection [RNA-seq_D8_JQ1]

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RNA-Seq of CD8+ T cell subsets during LCMV infection [RNA-seq_D8_Brd4_KO]

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Expression data from LCMV-infected P14 and Akt transgenic P14 CD8 T cells

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2012-03-01 | E-GEOD-36168 | biostudies-arrayexpress

RNA sequencing analysis of transferred C1qbp sufficient and deficient CD8+ T cells sorted from donor mice post infection with LCMV Armstrong

Project description:Tthis study is aimed to explore how C1qbp deficiency affects the mRNA expressing profiling during the differentiation stage of CD8+ T cells.Naïve WT and C1qbp KO P14 cells and transferred WT and C1qbp KO P14 cells sorted from the spleen of donor mice on day 3, 5, 7 post-infection with LCMV Armstrong were collected and treated for RNA-seq. RNA-Seq analysis revealed transcriptomic changes between WT and C1qbp KO CD8+ T cells. Naïve WT and C1qbp KO CD8+ T cells showed similar gene expressing profiling. On day 3, 5, 7 post-infection with LCMV Armstrong, WT and C1qbp KO CD8+ T cells showed 341, 1,164 and 437 differentially expressed genes respectively.

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Memory-like CD8 T-cells sustain the immune response to chronic viral infections.

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CXCR5+ Follicular Cytotoxic T cells Control Viral Infection in B Cell follicles

2016-07-31 | GSE76279 | GEO

CXCR5+ Follicular Cytotoxic T cells Control Viral Infection in B Cell follicles

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2016-07-31 | E-GEOD-76279 | biostudies-arrayexpress

Gene expression analysis of Wnt+ and Wnt- effector CD8 T cells

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2013-12-16 | E-GEOD-53358 | biostudies-arrayexpress

RNA-seq of LCMV GP33-specific CD8 TCR transgenic cells (P14) primed at the onset versus in the midst of persistent LCMV-Clone 13 viral infection

Project description:Altered CD8 T cell differentiation and functional exhaustion prevent control of chronic virus infection and cancer. Yet, how fate commitment and exhaustion are determined and dynamically modulated throughout persistent infection are unclear. We compared the activation and differentiation of LCMV GP33-specific CD8 TCR transgenic cells (P14) primed at the onset versus in the midst of established persistent LCMV-Clone 13 viral infection. LCMV GP33-specific CD8 TCR transgenic (P14) cells were injected into naïve mice immediately infected with LCMV-Cl13 (Early priming) or into mice that had been infected 21 days earlier with LCMV-Cl13 (Late Priming). Sixty hours post-priming P14 cells were sorted from mice and subjected to RNA seq. We show early primed cells very rapidly exhibit a transcriptional profile of robust activation, effector differentiation and dysfunction, while late primed cells have increased expression of genes involved in memory differentiation and maintenance.

2018-10-09 | GSE105044 | GEO

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