Adaptive local false discovery rate procedures for highly spiky data and their application to RNA sequencing data of yeast SET4 deletion mutants

ABSTRACT: Genome-wide gene expression data were genertated for wildtype and set4 mutant yeast to determine the contribution of Set4 to gene expression under standard growth conditions. Illumina-based sequencing was performed on strand-specific libraries prepared from mRNA purified from yeast under standard (YPD-rich medium, no stress) growth conditions. Reads were procesed for qualiy control, mapped to the genome, and differential gene expression was determined using based on log fold-change difference between set4 mutants and wildtype. The distribution of the data was highly spiky and an adaptive local false discovery rate analysis was applied to analyze these data.

ORGANISM(S): Saccharomyces cerevisiae

PROVIDER: GSE173653 | GEO | 2021/10/01

REPOSITORIES: GEO