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Massively parallel quantification of SpCas9 gRNA efficiencies by surrogate lentiviral libraries

ABSTRACT: In this study, we used a surrogate lentivirus library to capture CRISPR editing outcome in HEK cells. The dataset include quantification of indel frequencies for SpCas9 gRNAs in 12,000 surrogate sites. After filtering low quality sites, the high quality SpCas9 gRNA activities from a total of 10592 sites have been used to develop an improved deep learning-based prediction model CRISPRon (https://rth.dk/resources/crispr/.).

ORGANISM(S): Homo sapiens

PROVIDER: GSE173708 | GEO | 2021/05/03

REPOSITORIES: GEO

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