Dataset Information

Suceptibility of non target crustacean Eurytemora affinis to endocrine disruptor tebufenozide: a transcriptomic approach

ABSTRACT: Copepods are zooplanktonic crustaceans ubiquitously widespread in aquatic systems. They are of ecological importance due to their place in the trophic chain. Copepods are exposed to a wide variety of pollutants as insect growth regulators (IGR) acting as endocrine disruptors although they are not the target. The aim of the present work was to investigate the molecular response of a non-targeted organism, the copepod Eurytemora affinis, to an IGR. Adult males and females were exposed at two sub-lethal concentrations of tebufenozide (TEB), an agonist of the ecdysone receptor targeting lepidopteran larvae. Results suggest a gender-specific response with a higher sensitivity in males potentially due to a differential activation of stress response pathways. Indeed, genes coding for proteins involved in stress response were mis-regulated in males while not in females suggesting that detoxification processes are still ongoing in males. In both genders, TEB exposure triggers similar pathways as for its targeted species by modulating the transcription of early and late ecdysone responsive genes. Among them, genes involved in cuticle metabolism, muscle contraction, neurotransmission and gametogenesis whose mis-regulation could lead to moult, locomotor and reproductive impairments. Furthermore, genes coding for proteins playing part in epigenetic processes were found in both genders highlighting the potential impact of TEB exposure on further generations. Beyond the in-depth knowledge of the molecular MoA of an ecdysone agonist on a non-targeted organism, this work provides data allowing the identification of (i) potential biomarkers of ecdysone agonists and (ii) putative physiological responses to be further assessed to characterize the effects of TEB at higher biological levels. The present study reinforces the suitability of the use E. affinis as an ecotoxicological model.

ORGANISM(S): Eurytemora affinis

PROVIDER: GSE173927 | GEO | 2021/10/27

REPOSITORIES: GEO

ACCESS DATA

Similar Datasets

Project description:While women are more vulnerable than men to many of the medical consequences of alcohol abuse, the role of sex in the response to ethanol is controversial. Neuroadaptive responses that result in the hyperexcitability associated with withdrawal from chronic ethanol likely reflect gene expression changes. We have examined both genders for the effects of withdrawal on brain gene expression using mice with divergent withdrawal severity that have been selectively bred from a genetically heterogeneous population. A total of 295 genes were identified as ethanol regulated from each gender of each selected line by microarray analyses. Hierarchical cluster analysis of the arrays revealed that the transcriptional response correlated with sex rather than with the selected withdrawal phenotype. Consistent with this, gene ontology category over-representation analysis identified cell death and DNA/RNA binding as targeted classes of genes in females, while in males, protein degradation, and calcium ion binding pathways weremore altered by alcohol. Examination of ethanol regulated genes and these distinct signaling pathways suggested enhanced neurotoxicity in females. Histopathological analysis of brain damage following ethanol withdrawal confirmed elevated cell death in female but not male mice. The sexually dimorphic response was observed irrespective of withdrawal phenotype. Combined, these results indicate a fundamentally distinct neuroadaptive response in females compared to males during chronic ethanol withdrawal and are consistent with observations that female alcoholics may be more vulnerable than males to ethanol-induced brain damage associated with alcohol abuse. A total of 32 microarrays were run with 4 biological replicates per treatment, line, and sex. Selection replicates (i.e. WSP-1 and WSP-2) for each treatment, line, and sex were collapsed to improve statistical power (n=4) and to facilitate in the identification of phenotype related effects and exclude selection artifacts. For comparisons, EtOH regulation was determined by comparing 4 arrays from (for example) Male WSR EtOH treated versus 4 arrays from Male WSR Air treated arrays.

Dataset Information

Suceptibility of non target crustacean Eurytemora affinis to endocrine disruptor tebufenozide: a transcriptomic approach

Similar Datasets

OmicsDI is part of the ELIXIR infrastructure