Transcriptomics,Genomics

Dataset Information

41

Genome-wide comparison of EpiSC lines derived from fertilized (FT) and somatic cell nuclear transfer (NT) embryos


ABSTRACT: Genome wide comparison of gene expression between EpiSC lines derived from fertilized (FT) embryos and somatic cell nuclear transfer (NT) embryos. EpiSC lines were derived from fertilized and somatic cell nuclear transfer embryos and cultured until 15 to 20 passages. RNA was then extracted in order to compare transcriptomic profiles. Overall design: Total RNA was extracted from 3 FT-EpiSC lines, and 3 NT-EpiSC lines. For each line, there were 3 biological repeat.

INSTRUMENT(S): Illumina MouseWG-6 v2.0 expression beadchip

SUBMITTER: Julien MARUOTTI  

PROVIDER: GSE17402 | GEO | 2010-02-05

SECONDARY ACCESSION(S): PRJNA118849

REPOSITORIES: GEO

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Publications

Nuclear transfer-derived epiblast stem cells are transcriptionally and epigenetically distinguishable from their fertilized-derived counterparts.

Maruotti Julien J   Dai Xiang Peng XP   Brochard Vincent V   Jouneau Luc L   Liu Jun J   Bonnet-Garnier Amélie A   Jammes Hélène H   Vallier Ludovic L   Brons I Gabrielle M IG   Pedersen Roger R   Renard Jean-Paul JP   Zhou Qi Q   Jouneau Alice A  

Stem cells (Dayton, Ohio) 20100401 4


Mouse embryonic pluripotent stem cells can be obtained from the inner cell mass at the blastocyst stage (embryonic stem cells, ESCs) or from the late epiblast of postimplantation embryos (epiblast stem cells, EpiSCs). During normal development, the transition between these two stages is marked by major epigenetic and transcriptional changes including DNA de novo methylation. These modifications represent an epigenetic mark conserved in ESCs and EpiSCs. Pluripotent ESCs derived from blastocysts g  ...[more]

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