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Gene expression profile analysis of F2 cross between Fat and Lean chickens

ABSTRACT: The aim of this study was to identify genes involved in the variation of the muscle glycogen content at death (estimated through the glycolytic potential, GP), a determining factor in the meat quality of chicken. The gene expression profiles of the Pectoralis major muscle were established using microarrays. We compared animals showing extreme GP values, issued from a F2 cross between Fat and Lean lines. A total of 254 genes were differentially expressed between F2 birds with low or high GP. Several of these genes were validated by qRT-PCR. For the genes with human orthologs, annotation analyses were performed and mainly revealed pathways involved in carbohydrate, fatty-acid and protein metabolism. The relationship between gene expression and meat quality has to now be validated by further e-QTL studies on the F2 population.

ORGANISM(S): Gallus gallus

PROVIDER: GSE17428 | GEO | 2009/08/04

SECONDARY ACCESSION(S): PRJNA118899

REPOSITORIES: GEO

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Gene expression profile analysis of F2 cross between Fat and Lean chickens

Project description:The aim of this study was to identify genes involved in the variation of the muscle glycogen content at death (estimated through the glycolytic potential, GP), a determining factor in the meat quality of chicken. The gene expression profiles of the Pectoralis major muscle were established using microarrays. We compared animals showing extreme GP values, issued from a F2 cross between Fat and Lean lines. A total of 254 genes were differentially expressed between F2 birds with low or high GP. Several of these genes were validated by qRT-PCR. For the genes with human orthologs, annotation analyses were performed and mainly revealed pathways involved in carbohydrate, fatty-acid and protein metabolism. The relationship between gene expression and meat quality has to now be validated by further e-QTL studies on the F2 population. 8 high GP samples compared to 8 low GP samples, 4 of these were dye-swapped.

2010-06-24 | E-GEOD-17428 | biostudies-arrayexpress

Gene expression profile analysis of Fat and Lean chickens

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Gene expression profile analysis of Fat and Lean chickens

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Transcription profiling of pig muscle longissimus dorsi sample of f2 animals, T-test paired and SAM

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