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A single-cell genomic strategy for alternative TSSs identification [scTSS-seq]

ABSTRACT: Alternative transcription start sites (TSSs) usage plays a critical role in gene transcription regulation in mammals. However, precisely identifying alternative TSSs remains challenging at the genome-wide level. Here, we report a single-cell genomic technology for alternative TSSs annotation and cell heterogeneity detection. Here, we utilize Fluidigm C1 system to capture individual cells of interest, SMARTer cDNA synthesis kit to recover full-length cDNA, then dual priming oligonucleotide system to specifically enrich 5’-end tags for genomic analysis.

ORGANISM(S): Mus musculus

PROVIDER: GSE174479 | GEO | 2023/01/01

REPOSITORIES: GEO

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